Absorption flattening correction of circular dichroism spectra of particle suspensions

There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension.

Name dc:type.qualificationname

mphil

Level dc:type.qualificationlevel

masters-pg

Grantor dc:publisher.institution

University of Strathclyde

Year dc:date.issued

2013

Author dc:creator

• Gerdova, Anna

DOI

10.48730/rg1w-wq87

Identifier

T13569

OAI identifier oai:identifier

oai:strathclyde:zp38wc69f