{"id":{"repo_id":"strathclyde","oai_identifier":"oai:strathclyde:zp38wc69f"},"canonical_url":"https://search.dev.ndltd.org/etd/strathclyde/oai:strathclyde:zp38wc69f","repository":{"repo_id":"strathclyde","name":"University of Strathclyde","base_url":"https://stax.strath.ac.uk/catalog/oai"},"display":{"title":"Absorption flattening correction of circular dichroism spectra of particle suspensions","abstract":"There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension.","abstract_html":"There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension.","abstract_has_math":false,"creators":["Gerdova, Anna"],"institution":"University of Strathclyde","degree_name":"mphil","degree_level":"masters-pg","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013","date_published":"2013","updated_at":"2026-07-24T04:47:01Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["10.48730/rg1w-wq87"],"render_values":[{"text":"10.48730/rg1w-wq87","href":"https://doi.org/10.48730/rg1w-wq87","code":true}]},{"key":"dc:identifier","label":"Identifier","values":["T13569"],"render_values":[{"text":"T13569","href":null,"code":true}]}]},"links":{"outbound_url":"https://stax.strath.ac.uk/concern/theses/zp38wc69f","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gerdova, Anna"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013"]},{"key":"dc:date.issued","label":"Date","values":["2013"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Pure and Applied Chemistry"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Strathclyde"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["masters-pg"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["mphil"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["T13569"]},{"key":"dc:identifier.doi","label":"DOI","values":["10.48730/rg1w-wq87"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://stax.strath.ac.uk/concern/theses/zp38wc69f"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension."]},{"key":"dc:description.abstract","label":"Abstract","values":["There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension."]},{"key":"dc:title","label":"Title","values":["Absorption flattening correction of circular dichroism spectra of particle suspensions"]}]}],"canonical_facts":{"dc:creator":["Gerdova, Anna"],"dc:date":["2013"],"dc:date.issued":["2013"],"dc:description":["There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension."],"dc:description.abstract":["There are a number of theoretical models for correction for absorption flattening of circular dichroism (CD) measurements on particle suspensions. However, they have not been directly tested experimentally and there remains doubt as to whether the corrections are actually accurate. The aim of this thesis was to test the correction method recently developed by Halling (2009), by comparing corrected CD spectra with the expected ones. Proposed simulation method was applied to a test model containing different particles (both protein and non-protein nature). In case of nonprotein containing particles corrections seem to work, but for protein-containing samples corrected CD spectra did not agree with the expected ones. The alternative approach to solving the problem with effect of absorption flattening was tested. The use of intensive synchrotron source allowed collecting feasible CD spectra on single protein-containing particles (100 μm in diameter). This approach also revealed structural differences between particles in the same suspension."],"dc:identifier":["T13569"],"dc:identifier.doi":["10.48730/rg1w-wq87"],"dc:identifier.uri":["https://stax.strath.ac.uk/concern/theses/zp38wc69f"],"dc:publisher.department":["Department of Pure and Applied Chemistry"],"dc:publisher.institution":["University of Strathclyde"],"dc:title":["Absorption flattening correction of circular dichroism spectra of particle suspensions"],"dc:type.qualificationlevel":["masters-pg"],"dc:type.qualificationname":["mphil"]},"updated_at":"2026-07-24T04:47:01Z"}