Design and development of an electrochemical and infrared spectroscopic medical device for serum-based cancer diagnostics

Development of an integrated electrochemical and spectroscopic serum diagnosticdevice would have significant potential as a triage tool for brain cancer and Hodgkin’slymphoma in primary care settings to better inform clinical decisions and facilitateprompt referral to secondary care, whilst reducing financial and practical constraintsplaced on current diagnostic modalities. Additionally, the ability to detect IDH1molecular status from serum samples would have significant clinical value to patientsand clinicians alike, ultimately allowing earlier diagnosis and improved planning ofsurgery and treatment therapeutics. To this extent, the medical device hasdemonstrated the ability to electrochemically detect the biomarker CCL17/TARC at387-50,000pg/ml concentrations with R2 = 0.979 and limit of detection of 387pg/mlin spiked buffer samples. Thereafter, the medical device demonstrated successfuldiagnosis of Hodgkin’s lymphoma in all 11 tested clinical patient samples.Additionally, ATR-FTIR discriminated between Hodgkin’s lymphoma and healthycontrols in 200 patient samples with sensitivity of 83.2 ± 6.6% and specificity of 85.3± 8.1% with Random Forest classification, highlighting differences in proteinsecondary structures within clinical serum samples. The integrated diagnostic platformfurther demonstrated the ability to electrochemically detect IDH1-R132H proteins inspiked buffer samples at 0.05-10,000ng/ml concentrations with R2 = 0.958. However,it was not subsequently possible to detect IDH1 mutant proteins in clinical serumsamples. Nevertheless, ATR-FTIR demonstrated the ability to discriminate betweenIDH1 molecular status in 104 glioma patients through consideration of the globalmolecular signatures ofserum samples with sensitivity of 89.0 ± 11.3% and specificityof 88.2 ± 10.1% with PLS classification.

Name dc:type.qualificationname

engd

Level dc:type.qualificationlevel

doctoral-pg

Grantor dc:publisher.institution

University of Strathclyde

Year dc:date.issued

2020

Author dc:creator

• Rinaldi, Christopher

Advisors dc:contributor.advisor

• Baker, Matthew
• Dennany, Lynn

DOI

10.48730/51ne-0g92

Identifier

T15633

Author Identifier

201559983

OAI identifier oai:identifier

oai:strathclyde:2j62s4901