Investigation of preferential binding properties of monoclonal antibodies M1:1D2 and H4:4 towards the conformers of recombinant prion protein

Unlike bacteria and viruses which contain nucleic acids, prions are proteinaceous infectious agents. Prion diseases are clinically diagnosed by post-mortem histopathological examination of brain tissue and western blotting to detect proteinase K resistant prion protein. At present, no diagnostic test exists for the detection of prion diseases in live animals or humans. Use of mAbs will certainly increase sensitivity of diagnosis and it may also allow detection of PrPSc in body fluids. In this project the two mAbs M1:1D2 (IgG3) and H4:4 (IgG2a) which were raised against prion protein purified from outdated human platelets were tested for their specificity against SDS aggregated recombinant human PrP peptide. The mAbs were able to discriminate between aggregated and monomeric forms of recombinant PrP peptide and were found to be selective. These mAbs could perhaps be used in a diagnostic tool or an antibody assay against the infected or misfolded form of prion protein or PrPSc.

Name dc:type.qualificationname

mres

Level dc:type.qualificationlevel

masters-pg

Grantor dc:publisher.institution

University of Strathclyde

Year dc:date.issued

2010

Author dc:creator

• Nainawat, Jayant

DOI

10.48730/z7dr-3e37

Identifier

T12764

OAI identifier oai:identifier

oai:strathclyde:12579s31z