Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells

In order to localize replicating polyoma viral DNA (Py II*), a nuclear extraction technique is chosen and then slightly modified in order to rapidly yield purified nuclei in which the polyoma viral (Py) DNA is intact. A method is also developed which breaks the nuclear membrane, but not the Py DNA found within. As could be expected, both mature polyoma viral DNA (Py I) and Py II* are found primarily in the nucleus of Py-infected mouse embryo cells. Very little Py I or II* is found in the nucleolus or in the cytoplasm. These two forms of Py DNA are located in the same sub-nuclear fraction. At least half of each is not in association with the nuclear membrane. Possible locations for the rest of the Py DNA are discussed. A study of the localization of cellular DNA reveals that all is membrane associated. Two types of bonds are distinguished but only the first type is studied. It is found to depend on a non-covalent protein or lipoprotein-DNA interaction. Two very rapidly sedimenting polyoma DNA-protein complexes are isolated and partially characterized. The faster of the two could be a replicative complex, whereas the slower is comprised uniquely of Py I. This complex has properties that could lead to its being mistaken for a DNA-membrane association, and thus cautions against rapid identification o.f DNA membrane bonds.

Name thesis:degree_name

M. Sc.

Level thesis:degree_level

Maîtrise

Discipline thesis:degree_discipline

Microbiologie

Grantor dc:publisher

Université de Sherbrooke

Year dc:date.issued

1973

Author dc:creator

• Frost, Eric

Advisor dc:contributor.advisor

• Bourgaux, P.

• Virologie
• Virus
• Microbiologie

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/11143/18492

OAI identifier oai:identifier

oai:usherbrooke.scholaris.ca:11143/18492