Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"DNA-Protein Complexes"”.
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Cis and trans signals for the replication of bovine parvovirus
… homologus regions between these termini. Three proteins were identified that bind to the left terminal 171 nucleotides in the hairpin conformation. Inhibition of the formation of the DNA-protein complexes with competitor DNA localized two potential binding sites that correspond to the domains …
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Molecular mechanisms of the carcinogen chromium(VI)-induced DNA-protein crosslinking in cultured intact human cells
Chromium(VI)-induced DNA-protein crosslinking is implicated in chromium Carcinogenicity. However, the mechanism of chromate-induced DNA-protein crosslinking has not been established. Based on the current literature and our preliminary data, we hypothesized that Cr(VI)-induced DNA-protein crosslinks …
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Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells
In order to localize replicating polyoma viral DNA (Py II*), a nuclear extraction technique is chosen and then slightly modified in order to rapidly yield purified nuclei in which the polyoma viral (Py) DNA is intact. A method is also developed which breaks the nuclear membrane, but not the Py DNA …
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Molecular analysis of kinetochore-microtubule attachment in budding yeast
… during cell division. Kinetochores are DNA-protein complexes that assemble on centromeric DNA sequences. In budding yeast, the kinetochores consist of approximately fifty proteins that are organized into a large multi-subunit complex. Multiple kinetochore proteins are thought to work …
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Identification of Proteins Involved in Chloroplast DNA Replication
… <p>Chloroplast nucleoids (ct-nucleoids) are DNA/protein complexes involved in compacting the chloroplast genome, and may play a role in regulating DNA replication. Ct-nucleoids were isolated from young soybean plants and separated by 2-D gel electrophoresis. Gel spots were excised and …
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Solid-state nanopore sensors for nucleic acid analysis
Nanopore DNA analysis is an emerging technique that involves electrophoretically driving DNA molecules through a nano-scale pore in solution and monitoring the corresponding change in ionic pore current. This versatile approach permits the label-free, amplification-free analysis of charged polymers …
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Cellular factors and viral elements for parvovirus replication
… of the cellular S-phase factor, we performed DNA retardation assays with uninfected nuclear extracts of S-phase cells, synchronized by hydroxyurea pretreatment, and radiolabeled parvoviral termini in their hairpinned conformation. We observed that proteins in HeLa cells, a tissue culture host …
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Intact and Damaged DNA and their Interaction with DNA-Binding Proteins: a Single Molecule Approach
… we study the architecture of intact and damaged DNA and their interaction with DNA-binding proteins using a combination of atomic force microscopy (AFM) and fluorescence correlation spectroscopy (FCS). In the beginning of this scientific work we therefore focus our efforts on the development of a …
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Transcriptional regulation of the human alpha 2(I) procollagen gene
… runon transcription experiments . Analysis of DNA-protein interactions with the proximal α2(1) procollagen promoter showed the presence of at least two DNA-protein complexes (complexes I and III) in collagen producing cell lines, while cells where collagen synthesis did not occur contained a …
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Characterisation of CIS- and trans-acting factors that regulate the human alpha 2(1) procollagen gene
… has been correlated with differential in vitro DNA-protein interactions on the basal promoter region between -100 and -67. A 23 bp region of the a.2(1) procollagen promoter encompassing the G/CBE (CCTCCATTGG) and the Ctv'IE (GGAGGCCCTTTT) has previously been shown to engage in specific DNA …