Effect of cereal type and commensal bacteria on availability of methionine sources and intestinal physiology in pigs

An investigation was conducted to determine the contribution of the gastrointestinal microbiota to variation in bioefficacy of methionine sources and the interrelationship between intestinal microbiota and cereal grain type with respect to gastrointestinal physiology. Apparent gastrointestinal absorption of DL-methionine (MET) and 2-hydroxy-4-methylthiobutanoic acid (MHA-FA), post-weaning intestinal morphology, digestive physiology, mucin dynamics and digesta flow were studied in a series of experiments using conventional and gnotobiotic pigs. At 14 d of age, sow - reared conventional (CON) pigs and isolator - reared monoassociated gnotobiotic pigs (EF) were weaned to corn or wheat/barley based diets supplemented with MET or MHA-FA. At 24 d of age, after an overnight fast, pigs were fed experimental diet supplemented with 107 Bq of either 3H-L-MET or 3H-L-MHA-FA per kg of feed and chromic oxide (0.5% wt/wt). Pigs were killed 3 h after consuming the meal to collect digesta and tissue samples from the stomach and along the small intestinal (SI) length. Conventional pigs fed a wheat/barley-based diet had increased (P < 0.05) total aerobes, whereas supplementation with MHA-FA increased (P < 0.05) total aerobes and lactobacilli populations in proximal SI. Among the gnotobiotic pigs, 8 pigs (2 isolators) were monoassociated with a bacteria closely related to Providencia spp. and 16 pigs (4 isolators) were monoassociated with Enterococcus faecium (EF). Species of bacterial contaminant and diet composition did not affect residual MET or MHA-FA in digesta. Decreased (P < 0.05) apparent residual MET in digesta compared with MHA-FA in CON but not monoasscoiated pigs, along with significantly higher (P

Name thesis:degree_name

Doctor of Philosophy (Ph.D.)

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Animal and Poultry Science

Grantor

University of Saskatchewan

Year dc:date.issued

2009

Author dc:creator

• Malik, Gita

Advisor dc:contributor.advisor

• Van Kessel, Andrew A. G.

Committee members dc:contributor.committeemember

• Drew, Murray D.
• Nyachoti, Martin
• Laarveld, Bernard

• commensal microbiota
• digesta flow
• methionine hydroxy-analogue
• mucin

Language dc:language.iso

en_US

Handle dc:identifier.uri

https://hdl.handle.net/10388/etd-09182009-124202

OAI identifier oai:identifier

oai:harvest.usask.ca:10388/etd-09182009-124202