Reguläre bakterielle Zellhüllenproteine als biomolekulares Templat

Bacterial cell wall proteins (S-layer) are - due to both the capability to self-assemble into two-dimensional crystals and their distinct chemical and structural properties - suitable for the deposition of metallic particles at their surface . The cluster growth is subject of this thesis. The binding of metal complexes to S-layers of Bacillus sphaericus and Sporosarcina ureae and their subsequent reduction leads to the formation of regularly arranged platinum or palladium cluster arrays on the biomolecular template. A heterogeneous nucleation mechanism is proposed for this process consisting of the binding of metal complexes and their subsequent reduction. The kinetics of the process and the binding of the complexes to the protein are characterized by UV/VIS spectroscopy. This thesis focuses on structural investigations by means of transmission electron microscopy, electron holography, scanning force microscopy, image analysis, and image processing. Preferred cluster-deposition sites are determined by correlation averaging. A more precise determination and quantification is obtained by Multivariate Statistical Analysis. Furthermore a method for the electron beam induced formation of highly-ordered metallic cluster arrays in the transmission electron microscope and a fast screening method for surface layers of Gram-positive bacteria are presented.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Technische Universität Dresden

Year

2003

Author dc:creator

• Wahl, Reiner

Contributors dc:contributor

• Lichte, Hannes
• Baumeister, Wolfgang
• Pompe, Wolfgang

• Bildanalyse
• Bildverarbeitung
• Biotemplating
• Reguläre bakterielle Zellhüllenproteine
• S-Layer
• Transmissionselektronenmikroskopie
• Bacterial cell wall proteins
• Image analysis
• Image processing
• Transmission electron microscopy