Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"S-Layer"”.
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Bioengineering of S-layers: molecular characterization of the novel S-layer gene sslA of Sporosarcina ureae ATCC 13881 and nanotechnology application of SslA protein derivatives
S-layer proteins of S. ureae ATCC 13881 form on the cell surface an S-layer lattice with p4 square type symmetry and a period of about 13.5 nm. These lattices were shown to be the excellent nanotemplates for deposition of regular metal clusters. The synthesis of the S. ureae S-layer protein is …
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Synthetic scaffolds and protein assemblies for engineering applications
S-layer proteins, which naturally self-assemble on the exterior of cells, provide an interesting basis for the creation of synthetic scaffolds. In this thesis, I created a plasmid which produces a recombinant form of a well characterized S layer protein, sbpA, which has a number of properties ideal …
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Development and properties of tracheids and their cell wall layers in eastern hemlock (Tsuga canadensis L.)
… and chemical properties of individual cell wall layers. Results showed that 59% of the middle lamella and primary wall was lignin, but these layers contained only 19% of the cells total lignin content. Lignin content of the secondary wall was higher in the S<sub>3</sub> layer, (35%) than in the …
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Electrochemical nanomoulding through proteins
… fabrication.Bacterial cell-surface layer proteins, or "S-layer" proteins, from two organisms---Deinococcus radiodurans and Sporosarcina ureae---were used as the "moulds" for electrochemical fabrication. The proteins are easily purified as micron-sized sheets of periodic molecular …
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Mechanistic interactions between Sulfolobus islandicus and its viruses
… I share preliminary data on an evolved S-layer deficient host, ΔslaA_evol, which was created to test viral susceptibility and reveals that the S-layer is not the primary receptor for many viruses. In this thesis, I describe my work characterizing virus-host mechanisms to enhance our …
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S-Schichtproteine als molekulare Bausteine zur Funktionalisierung mikroelektronischer Sensorstrukturen
… interpretiert. Abstract* Bacterial surface layer proteins (S-layer proteins) can be used as molecular building blocks for the functionalisation of technically-used surfaces. For example, the proteins serve as templates for the production of well-defined patterns or provide binding sites for …
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Dynamic Regulation of Metabolism in Archaea
… of sugars incorporated into the cell surface S-layer glycoprotein. Because S-layer glycosylation is proportional to growth, we hypothesize that TrmB transduces a growth rate signal to co-regulated metabolic pathways including amino acid, purine, and cobalamin biosynthesis. Taken together, our …
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Phenotypic and genotypic characterisation of Clostridium Difficile
… minimum inhibitory concentrations(MICs) and S-layer protein typing. In addition to this, isolates were also investigated for the production of a range of extracellular enzymes as potential virulence factors. Genotypic characterisation was performed using a random amplification of polymorphic …
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Reguläre bakterielle Zellhüllenproteine als biomolekulares Templat
Bacterial cell wall proteins (S-layer) are - due to both the capability to self-assemble into two-dimensional crystals and their distinct chemical and structural properties - suitable for the deposition of metallic particles at their surface . The cluster growth is subject of this thesis. The …
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Identification and characterization of a novel capsule-like complex surface antigen of Francisella tularensis
Francisella tularensis is a highly virulent zoonotic pathogen that is the causative agent of tularemia in humans. Two subspecies of F. tularensis are the most virulent in humans: tularensis (type A) and holarctica (type B), with less than 10 organisms via aerosol of a type A strain having the …
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Fluxon Dynamics In Two-Gap Superconductor-Based Long Josephson Junction
… channels between the superconductor (S) layers and the inter-band Josephson effect within the same S layer. The inter-band Josephson effect leads to both spatial and temporal modulation of the critical current between the two adjacent S layers. In this work, the effects of critical …
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Continental Arc Processes in British Columbia and Earthquake Processes in Virginia: Insights from Seismic Imaging
… a high-velocity (P 7.0 km/s, S 3.8 km/s) layer in the lower crust beneath the youngest (late Cretaceous to Eocene) portion of the continental arc complex. In contrast, the lower crust under the Stikine terrane has lower velocities consistent with amphibolite or other hydrated mafic rocks. …
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Characterization of lignin deposition in <i>Pinus taeda</i> L. cell suspension cultures
… cells was consistent with secondary (S₁) layer formation. Despite the possibility of only limited lignin deposition in the 2,4-0 grown cells, secondary metabolism had occurred as evidenced by reversed-phase and chiral chromatographic separations which revealed the ability of these cells to …
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Roles of Small Rnas and Paralogous Proteins In Bacillus Anthracis Virulence Gene Regulation
… two genes encoding components of the surface layer (S-layer), a protein layer found between the cell wall and capsule which serves as a mounting structure for key surface-associated proteins. Genomic and transcriptomic studies identified a 70% identical paralog of PagR1, termed PagR2, found in …
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Clostridium difficile: expression of virulence factors, resistance to disinfectants and interactions with human cells
… Macrophages were challenged in vitro with S-layer proteins, flagella, heat-shock proteins and culture supernatants of the five strains and cytokine production was measured by specially developed ELISAs. No significant inter-strain differences were observed, although the epidemic strains …
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Novel Cell Surface Anchoring Mechanism Of Prokaryotic Secreted Protein
… well-studied model archaeon Haloferax volcanii S-layer glycoprotein (SLG), previously thought to be anchored in the membrane via its C-terminal hydrophobic domain, I have shown that its H-domain instead is processed and that this processing is dependent on a novel enzyme, the archaeosortase …
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Caracterização fisiológica e genética do Lactobacillus delbrueckii UFV H2b20 desprovido da capacidade de imunoestimulação
Foi feita a caracterização de um mutante espontâneo do Lactobacillus delbrueckii UFV H2b20 que mostrou-se desprovido da capacidade de imunoestimulação e também a investigação do possível papel da seqüência de inserção ISLdH2b20 nessa mutação. A confirmação da identidade genética de L. delbrueckii …
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Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay
… enolase (<em>eno</em>), S-layer protein (<em>ahsA</em>), serine protease (<em>ser), </em>Type IV <em>Aeromonas</em> pilus (<em>tap</em><em>A</em>), lipase (<em>lip</em>), and Type Three Secretion System (T3SS) components (<em>aopB</em>, <em>ascV</em>). Genes for …
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Seismic constraints on shallow crustal processes at the East Pacific Rise
… thin (<60 m) surficial low-velocity (<2.5 km/s) layer, a 100-150 m thick transition zone with velocities increasing by approximately 2.5 km/s, and a layer with velocities of ~5 km/s at a depth below the seafloor of 130-190 m. The surficial low-velocity layer and transition zone are defined as …
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Rheological and Thermal Insights into Solidification Dynamics in Extrusion-Based Additive Manufacturing of Polymer Composites
… 3D parts by depositing liquified material layer by layer, which solidifies to form a solid structure. Its key advantages over traditional methods like injection molding include design flexibility, the ability to create complex geometries (e.g., lattices, internal channels), and faster …