QUB-1439: A bioactive peptide from the frog skin secretion of Hylarana guentheri

Antimicrobial peptides (AMPs) are a sufficient and diverse group of molecules that are derive from various invertebrate, plant and animal species. AMPs possess a variety of biological activity, such as anticancer, antivirus and so on. Nowadays, a growing number of AMPs are considered as promising candidate of new drug. <br/><br/>This thesis focused on the antimicrobial peptide (QUB-1439) which was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 was synthesised by Tribute peptide synthesiser. Then, MALDI-TOF was used to check the molecular weight of the synthetic peptide. The crude peptide was purified by RP-HPLC. Finally, a series assays were performed to test the bioactivities of purified QUB-1439, including antimicrobial assay, anticancer assay and haemolysis assay. The results showed that QUB-1439 has ability to inhibit the growth of Gram-positive bacteria, the MIC of QUB-1439 against S. aureus is 16 μM. QUB-1439 does not exhibit toxicity and it has a weak effect on inhibition of cancer cell growth. Because QUB-1439 has these promising properties, it could be developped into a novel drug.<br/>

Name dc:type.qualificationname

Master of Philosophy

Level dc:type.qualificationlevel

Masters Thesis

Grantor dc:publisher.institution

Queen's University Belfast

Year dc:date.issued

2018

Author dc:creator

• Ling, Ziyu

Advisors dc:contributor.advisor

• Chen, Tianbao
• Wang, Lei
• Zhou, Mei
• Ma, Chengbang
• Shaw, Christopher

Language dc:language

eng

Identifier

oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97

OAI identifier oai:identifier

oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97