{"id":{"repo_id":"qu-belfast","oai_identifier":"oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97"},"canonical_url":"https://search.dev.ndltd.org/etd/qu-belfast/oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97","repository":{"repo_id":"qu-belfast","name":"Queen's University Belfast","base_url":"https://pureadmin.qub.ac.uk/ws/oai"},"display":{"title":"QUB-1439: A bioactive peptide from the frog skin secretion of Hylarana guentheri","abstract":"Antimicrobial peptides (AMPs) are a sufficient and diverse group of molecules that are derive from various invertebrate, plant and animal species. AMPs possess a variety of biological activity, such as anticancer, antivirus and so on. Nowadays, a growing number of AMPs are considered as promising candidate of new drug. <br/><br/>This thesis focused on the antimicrobial peptide (QUB-1439) which was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 was synthesised by Tribute peptide synthesiser. Then, MALDI-TOF was used to check the molecular weight of the synthetic peptide. The crude peptide was purified by RP-HPLC. Finally, a series assays were performed to test the bioactivities of purified QUB-1439, including antimicrobial assay, anticancer assay and haemolysis assay. The results showed that QUB-1439 has ability to inhibit the growth of Gram-positive bacteria, the MIC of QUB-1439 against S. aureus is 16 μM. QUB-1439 does not exhibit toxicity and it has a weak effect on inhibition of cancer cell growth. Because QUB-1439 has these promising properties, it could be developped into a novel drug.<br/>","abstract_html":"Antimicrobial peptides (AMPs) are a sufficient and diverse group of molecules that are derive from various invertebrate, plant and animal species. AMPs possess a variety of biological activity, such as anticancer, antivirus and so on. Nowadays, a growing number of AMPs are considered as promising candidate of new drug. &lt;br/&gt;&lt;br/&gt;This thesis focused on the antimicrobial peptide (QUB-1439) which was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 was synthesised by Tribute peptide synthesiser. Then, MALDI-TOF was used to check the molecular weight of the synthetic peptide. The crude peptide was purified by RP-HPLC. Finally, a series assays were performed to test the bioactivities of purified QUB-1439, including antimicrobial assay, anticancer assay and haemolysis assay. The results showed that QUB-1439 has ability to inhibit the growth of Gram-positive bacteria, the MIC of QUB-1439 against S. aureus is 16 μM. QUB-1439 does not exhibit toxicity and it has a weak effect on inhibition of cancer cell growth. Because QUB-1439 has these promising properties, it could be developped into a novel drug.&lt;br/&gt;","abstract_has_math":false,"creators":["Ling, Ziyu"],"institution":"Queen's University Belfast","degree_name":"Master of Philosophy","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chen, Tianbao","Wang, Lei","Zhou, Mei","Ma, Chengbang","Shaw, Christopher"],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-8","date_published":"2018-8","updated_at":"2026-07-24T03:54:53Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97"],"render_values":[{"text":"oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.qub.ac.uk/en/studentTheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chen, Tianbao","Wang, Lei","Zhou, Mei","Ma, Chengbang","Shaw, Christopher"]},{"key":"dc:creator","label":"Author","values":["Ling, Ziyu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-8"]},{"key":"dc:date.issued","label":"Date","values":["2018-8"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Pharmacy"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Queen's University Belfast"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.qub.ac.uk/en/studentTheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Master of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights.embargodate","label":"Dc Rights Embargodate","values":["2023-09-30"]},{"key":"dc:rights.embargoreason","label":"Dc Rights Embargoreason","values":["/dk/atira/pure/core/document/studentthesisembargoreason/publicationissues"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.qub.ac.uk/portal:studenttheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97","https://pure.qub.ac.uk/en/studentTheses/43b7cdf9-a033-4f5b-afae-cd62ffb22c97"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.qub.ac.uk/files/157526318/MPhil_Thesis_Ziyu_Ling.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Antimicrobial peptides (AMPs) are a sufficient and diverse group of molecules that are derive from various invertebrate, plant and animal species. AMPs possess a variety of biological activity, such as anticancer, antivirus and so on. Nowadays, a growing number of AMPs are considered as promising candidate of new drug. <br/><br/>This thesis focused on the antimicrobial peptide (QUB-1439) which was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 was synthesised by Tribute peptide synthesiser. Then, MALDI-TOF was used to check the molecular weight of the synthetic peptide. The crude peptide was purified by RP-HPLC. Finally, a series assays were performed to test the bioactivities of purified QUB-1439, including antimicrobial assay, anticancer assay and haemolysis assay. The results showed that QUB-1439 has ability to inhibit the growth of Gram-positive bacteria, the MIC of QUB-1439 against S. aureus is 16 μM. QUB-1439 does not exhibit toxicity and it has a weak effect on inhibition of cancer cell growth. Because QUB-1439 has these promising properties, it could be developped into a novel drug.<br/>"]},{"key":"dc:title","label":"Title","values":["QUB-1439: A bioactive peptide from the frog skin secretion of Hylarana guentheri"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chen, Tianbao","Wang, Lei","Zhou, Mei","Ma, Chengbang","Shaw, Christopher"],"dc:creator":["Ling, Ziyu"],"dc:date":["2018-8"],"dc:date.issued":["2018-8"],"dc:description.abstract":["Antimicrobial peptides (AMPs) are a sufficient and diverse group of molecules that are derive from various invertebrate, plant and animal species. AMPs possess a variety of biological activity, such as anticancer, antivirus and so on. Nowadays, a growing number of AMPs are considered as promising candidate of new drug. <br/><br/>This thesis focused on the antimicrobial peptide (QUB-1439) which was isolated from the skin secretion of Hylarna guentheri. It is the first time to identify the biosynthetic precursor encoding cDNA of QUB-1439 from the skin secretion-derived cDNA library though the mature peptide sequence, FLPLLFGAISHLL-NH2, has been identified before. Subsequently, QUB-1439 was synthesised by Tribute peptide synthesiser. Then, MALDI-TOF was used to check the molecular weight of the synthetic peptide. The crude peptide was purified by RP-HPLC. Finally, a series assays were performed to test the bioactivities of purified QUB-1439, including antimicrobial assay, anticancer assay and haemolysis assay. The results showed that QUB-1439 has ability to inhibit the growth of Gram-positive bacteria, the MIC of QUB-1439 against S. aureus is 16 μM. QUB-1439 does not exhibit toxicity and it has a weak effect on inhibition of cancer cell growth. 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