Analysis of the role of nuclear factor-kappa B in insulin resistance caused by antiretroviral drugs

Human immunodeficiency virus still remains the leading cause of death globally including women of child-bearing age. The rate of AIDS-related death has significantly declined since the introduction of antiretroviral treatment and other non-medical interventions such as the distribution and use of condoms. The introduction of antiretroviral treatment has however led to insulin resistance amongst users. Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated nuclease 9 (Cas) has been used to knockout NFκB to understand the pathway at which antiretroviral treatment causes insulin resistance. Heteroduplex mobility assay has shown that CRISPR-Cas9 knock out the gene of interest. These results have played a foundation in understanding how CRISPR-Cas9 can be integrated and utilized in medical research.

Grantor dc:publisher

University of Pretoria

Year dc:date.issued

2020

Advisor dc:contributor.advisor

• Pillay, Tahir S.

• UCTD
• Insulin resistance
• HAART
• CRISPR-Cas9
• NFkB
• Diabetes
• HIV/AIDS treatment
• Antiretroviral drugs
• Nuclear factor-Kappa B (NF-κB)
• Cytokines
• Adipose tissue
• Immune response

Statement dc:rights

• © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Language dc:language.iso

en

Dc Identifier Other

A2021

OAI identifier oai:identifier

oai:repository.up.ac.za:2263/79321