Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"clustered regularly interspaced"”.
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Analysis of the role of nuclear factor-kappa B in insulin resistance caused by antiretroviral drugs
… however led to insulin resistance amongst users. Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated nuclease 9 (Cas) has been used to knockout NFκB to understand the pathway at which antiretroviral treatment causes insulin resistance. Heteroduplex mobility assay …
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An expanded search for RNA-programmable genomic engineering effectors
… metagenomic datasets for novel Class 2 CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) single effectors, akin to the revolutionary genome-engineering tools Cas9 and Cpf1. Whereas previous search strategies relied on protein proximity to CRISPR-associated spacer acquisition …
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Identification of genotype-specific dependencies in Keap1-deficient lung adenocarcinoma
… of somatic genome editing provided by clustered regularly interspaced short palindromic repeats (CRISPR) and the endonuclease, CRISPR associated protein 9 (Cas9) has accelerated our ability to functionally interrogate putative genetic drivers in the context of mammalian cells and …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… activator-like effector nucleases) and CRISPR (clustered regularly interspaced short paindromic repeats). In contrast to inactivation approaches, there are approaches such as activating mutagenesis and ectopic expression, through which we increase the expression. In addition, gene expression and …
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Genomic nucleic acid memory storage with directed endonucleases
… of the bacterial immune system that consists of Clustered Regularly Interspaced Short Palindromic Repeat Sequences (CRISPR), which can recognize and cleave viral DNA using an RNA-guided directed endonuclease. Cuts left by the endonuclease are repaired by an error-prone DNA damage repair …
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Decipher in situ signaling and complex genetics with cellular recording and combinatorial perturbations
… cellular behaviors. The recent discovery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) system from prokaryotic adaptive immune system demonstrated unprecedented genome editing efficiency and programmability in sequence specific genome editing of …
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CRISPR3 Regulates Exopolysaccharide Production in Myxococcus xanthus
… and Type IV pili (T4P), harbors three Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) on its chromosome. CRISPR systems, which function as an adaptive immune system in prokaryotes, are classified into three types based on CRISPR-associated genes. Evidence suggests …
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Mechanisms of bacterial CRISPR immunity and a phage counter-defense
… of defense is the adaptive immune system CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). Upon infection, the bacterial host uptakes a genetic memory from the bacteriophage to incorporate into its genome. This molecular memory is used to target CRISPR-associated (Cas) proteins …
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Genome editing with CRISPR-Cas9 in the Illinois long term selection experiment
Recent advances in genome editing by Clustered Regularly Interspaced Palindromic Repeat (CRISPR) and CRISPR Associated Protein 9 (Cas9) have markedly increased our ability to characterize genes and use genetics to the benefit of agriculture. In this work, we utilize this technology to study the …
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Discovery and characterization of Cas13b, a differentially regulated RNA-targeting CRISPR system
… RNA in a simple, efficient manner. CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) systems, which confer adaptive immunity to prokaryotes, have revolutionized DNA targeting through the engineering of RNA-programmable Cas9-based tools. …
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Methods for CRISPR Cas12a Multiplexing in Mammalian Systems
Clustered regularly interspaced short palindromic repeat (CRISPR) proteins have been found in many bacterial species, serving as a defense mechanism to defend the cell from bacteriophage invasion. These proteins are guided to cleave a specific nucleic acid sequence through the binding of a crRNA …
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An integrated CRISPR-Cas toolkit for engineering human cells
Natively functioning Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated (Cas) system is a prokaryotic adaptive immune system that confers resistance to foreign genetic elements including plasmids and phages. Very recently, a two-component CRISPR-Cas technology from …
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Investigating Hybrid Cyclic/Linear and Linear Peptides as Vehicles for Nucleic Acid Delivery
<p>Small interfering RNA (siRNA) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated Protein 9 (Cas9) are advanced biotechnological approaches that have been able to temporarily downregulate gene expression through post-transcription RNA interference or …
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Investigating the transcriptional regulation of BCL11A in Triple-negative Breast Cancer
… a novel proteomic approach utilising the Clustered regularly interspaced short palindromic repeats – CRISPR associated 9 (CRISPR-Cas9) technology, to identify putative transcriptional regulators of BCL11A in TNBC. The first step of this project involves the identification of regulatory …
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Identification of novel genetic vulnerabilities and therapeutic targets in acute myeloid leukaemia using CRISPR dropout screens
… targets in AML, I have optimized a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screening platform and use it to identify genetic vulnerabilities in AML cells. This work led to the identification of 492 AML-specific cell-essential genes, including several …
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Cell-free synthetic biology for affordable, on-demand diagnostics
… Finally, I present work to develop and use clustered regularly interspaced short palindromic repeats (CRISPR) enzyme-based diagnostics to achieve attomolar sensitivity and single-nucleotide mismatch specificity. Together, these projects demonstrate a set of advancements in cell-free …
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Prevention of Duchenne Muscular Dystrophy by CRISPR/Cas Therapeutic Genome Editing
… for this debilitating disease. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) was first discovered as an adaptive immune system in bacteria and archaea for defending against phage infection. Recently, the CRISPR/Cas system has been …
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Comparative genomic approaches for investigating evolution in the microbial and viral populations of the rumen
… genomes, there were several mobile elements and clustered regularly interspaced short palindromic repeat (CRISPR) sequences detected, both of which indicate interactions with bacteriophages. The rumen virome sequences revealed a great deal of diversity in the viral populations. Additionally, the …
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Targeted destruction of intracellular DNA using a CRISPR-based genetic device that can be carried indefinitely in the host genome
… based on the organism's native type-IE CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) DNA interference (DNAi) pathway. The optimized DNAi device is comprised of two components: a chromosomally-integrated actuator element, which encodes the minimal set of CRISPR-associated …
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Applications of genome editing for disease modeling in mice
… endonuclease Cas9 from microbial type II CRISPR (clustered regularly interspaced short palindromic repeat) systems has been harnessed to facilitate facile genetic manipulations in a variety of cell types and organisms. Cas9 can be easily reprogrammed using RNA guides to generate targeted DNA …
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