SPATIOTEMPORAL CONTROL OF THE SALT STRESS INDUCED TRANSCRIPTIONAL RESPONSE IN ARABIDOPSIS

My study is focused on how different signaling pathways are integrated spatiotemporally under salt stress and how transcriptional regulation occurs. Firstly, I did an analysis on a previously generated spatiotemporal transcriptional map of salt stress in Arabidopsis roots, covering 4 core cell types and 6 time points for salt treatment. Based on this spatiotemporal map, the transcriptional changes of key components in hormone biosynthesis and signaling were identified, and a bioinformatics method was also developed to systematically de-convolve the hormone crosstalk network with salt stress, identifying some salt stress response sub-modules controlled by hormone signaling. Second, I developed an efficient pipeline to study the biological functions of cis-regulatory elements which are good starting points for the generation of a CRE centered transcriptional network involved in the salt stress response in the Arabidopsis roots. Using this pipeline, I validated the regulatory functions of seven CREs and two other previously unknown elements.

Author dc:creator

• WU RUI

• Arabidopsis root, salt stress, transcriptional regulation, spatiotemporal, CRE, synthetic promoter,