HEPATIC DIFFERENTIATION OF HUMAN AMNIOTIC EPITHELIAL CELLS AND IN VIVO THERAPEUTIC EFFECT ON ANIMAL MODEL OF CIRRHOSIS

HUMAN AMNIOTIC EPITHELIAL CELLS (HAECS) HAVE BEEN TOUTED AS AN IDEAL CANDIDATE FOR HEPATOCYTE DERIVATION. AFTER IN VITRO HEPATIC DIFFERENTIATION, HAEC-HEP CELLS SHOWED DIFFERENTIAL REGULATION IN GENES INVOLVED IN LIVER FUNCTION, CYP450 ACTIVITIES AND LIVER TRANSCRIPTION FACTORS. IMMUNOFLUORESCENCE STAINING SHOWED POSITIVITY FOR LIVER SPECIFIC MARKERS. FUNCTIONALLY, THESE CELLS SECRETED ALBUMIN, EXHIBITED INDUCIBLE CYP2C9 AND CYP3A4 ENZYMATIC ACTIVITIES, WERE ABLE TO UPTAKE AND CLEAR INDOCYANINE GREEN, AND UPTAKE LOW-DENSITY LIPOPROTEIN. AFTER CELLULAR TRANSPLANTATION INTO NSG MICE WITH THIOACETAMIDE INDUCED CHRONIC LIVER FAILURE, HAECS SHOWED ENGRAFTMENT AND CONTINUED TO SURVIVE ONE MONTH POST TRANSPLANTATION. LIVERS OF ANIMALS TRANSPLANTED WITH HAECS WERE LESS NODULAR AND LESS SHRUNKEN COMPARED TO CONTROLS. HAEC TRANSPLANTATION RESULTED IN SIGNIFICANT REDUCTION IN HEPATIC FIBROSIS AND IMPROVEMENTS IN LIVER FUNCTIONS. THIS OVERALL AUGMENTATION OF LIVER FAILURE WAS MEDIATED BY THE IN

Author dc:creator

• LIN SIQI JAYMIE

• Liver, fibrosis, amniotic membrane, hepatic differentiation, chronic liver failure