Northern Michigan University
BASIGIN-2 MEDIATED ACTIVATION OF ERK1/2 SIGNALING IN HUMAN GLIOBLASTOMA MULTIFORME CELLS
Abstract
dc:description.abstract<p>Glioblastoma multiforme (GBM) is the most common malignant form of human brain cancer. GBM tumor cells overexpress the protein Basigin (Bsg) at the cell surface where it contributes to malignancy via stimulation of matrix metalloproteinase (MMP) expression in surrounding normal tissues, resulting in the degradation of the extracellular matrix (ECM) surrounding tumors, promoting remodeling of the tumor borders, stimulating growth. In work by Belton et al. (2008), human uterine endometrial cells treated with a recombinant form of human basigin possessing the extracellular domain of the Bsg protein (rBsg-ECD) showed activation of the Mitogen-Activated Protein Kinase (MAPK) signaling pathway proteins, ERK1/2. This effect was mediated by rBsg-ECD binding to the Basigin-2 (Bsg-2) at the cell surface. In this research, U87-MG human GBM cells were treated with purified rBsg-ECD protein to measure changes in the phosphorylation of the ERK1/2 proteins. The results indicate the presence of a signaling loop within GBM tumors where soluble Bsg protein stimulates signal transduction through Bsg-2 at the cell surface. rBsg-mediated ERK1/2 stimulation is inhibited by the antioxidant compound Resveratrol, suggesting that the signaling mechanism through Bsg-2 involves the Epidermal Growth Factor Receptor (EGFR). Taken together, these results indicate that soluble Basigin protein stimulates signaling events through the MAPK signaling pathway by binding to Bsg-2 on the surface of GBM cells.</p>
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- Thesis
- Discipline thesis:degree_discipline
- Biology
- Year dc:date.available
- 2017
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Peterson, Erik R
- Contributors dc:contributor
-
- Dr. Robert Belton
Subjects
dc:subject × 8Identifiers
dc:identifier.*- Repository record dc:identifier
- https://commons.nmu.edu/theses/159
- OAI identifier oai:identifier
- oai:commons.nmu.edu:theses-1175