Evaluation of a Multiplex Real-Time PCR Assay to Simultaneously Detect Streptococcus pneumoniae and Staphylococcus aureus in Nasal Samples

<p>Invasive disease caused by <em>Streptococcus pneumoniae</em> and <em>Staphylococcus aureus</em> is associated with nasal carriage of these bacteria. Use of a pneumococcal conjugate vaccine (PCV) in children altered nasal carriage rates and reduced infections due to <em>S. pneumonia. </em>Similar studies demonstrate that there is an inverse correlation between <em>S. pneumoniae</em> and <em>S. aureus</em> nasal carriage in children, but this inverse correlation has not been extensively investigated adults. The PCV is now approved for use in adults, a group more likely to be colonized with <em>S. aureus</em>. To investigate the impact of PCV vaccination on <em>S. aureus</em> carriage in adults, the clinical evaluation of a multiplex Real Time PCR method to simultaneously detect very low levels of <em>S. pneumoniae</em> and <em>S. aureus</em> from the same nasal sample is described.</p>

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Clinical Sciences

Year dc:date.available

2016

Author dc:creator

• Hockberger, Polly

Contributors dc:contributor

• Paul Mann

• Multiplex
• Real-Time PCR
• Streptococcus pneumoniae
• Staphylococcus aureus
• Nasal
• Other Analytical, Diagnostic and Therapeutic Techniques and Equipment

Repository record dc:identifier

https://commons.nmu.edu/theses/117

OAI identifier oai:identifier

oai:commons.nmu.edu:theses-1135