Invasive disease caused by Streptococcus pneumoniae and Staphylococcus aureus is associated with nasal carriage of these bacteria. Use of a pneumococcal conjugate vaccine (PCV) in children altered nasal carriage rates and reduced infections due to S. pneumonia. Similar studies demonstrate that there is an inverse correlation between S. pneumoniae and S. aureus nasal carriage in children, but this inverse correlation has not been extensively investigated adults. The PCV is now approved for use in adults, a group more likely to be colonized with S. aureus. To investigate the impact of PCV vaccination on S. aureus carriage in adults, the clinical evaluation of a multiplex Real Time PCR method to simultaneously detect very low levels of S. pneumoniae and S. aureus from the same nasal sample is described.
","abstract_html":"<p>Invasive disease caused by <em>Streptococcus pneumoniae</em> and <em>Staphylococcus aureus</em> is associated with nasal carriage of these bacteria. Use of a pneumococcal conjugate vaccine (PCV) in children altered nasal carriage rates and reduced infections due to <em>S. pneumonia. </em>Similar studies demonstrate that there is an inverse correlation between <em>S. pneumoniae</em> and <em>S. aureus</em> nasal carriage in children, but this inverse correlation has not been extensively investigated adults. The PCV is now approved for use in adults, a group more likely to be colonized with <em>S. aureus</em>. To investigate the impact of PCV vaccination on <em>S. aureus</em> carriage in adults, the clinical evaluation of a multiplex Real Time PCR method to simultaneously detect very low levels of <em>S. pneumoniae</em> and <em>S. aureus</em> from the same nasal sample is described.</p>","abstract_has_math":false,"creators":["Hockberger, Polly"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Clinical Sciences","degree_department":null,"school":null,"contributors":["Paul Mann"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-12-01T08:00:00Z","date_published":"2016-12-01T08:00:00Z","updated_at":"2026-07-24T03:23:47Z","subjects":["Multiplex","Real-Time PCR","Streptococcus pneumoniae","Staphylococcus aureus","Nasal","Other Analytical, Diagnostic and Therapeutic Techniques and Equipment"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.nmu.edu/theses/117","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Paul Mann"]},{"key":"dc:creator","label":"Author","values":["Hockberger, Polly"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2016-12-03T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Clinical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Multiplex","Real-Time PCR","Streptococcus pneumoniae","Staphylococcus aureus","Nasal","Other Analytical, Diagnostic and Therapeutic Techniques and Equipment"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.nmu.edu/theses/117"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Invasive disease caused by Streptococcus pneumoniae and Staphylococcus aureus is associated with nasal carriage of these bacteria. Use of a pneumococcal conjugate vaccine (PCV) in children altered nasal carriage rates and reduced infections due to S. pneumonia. Similar studies demonstrate that there is an inverse correlation between S. pneumoniae and S. aureus nasal carriage in children, but this inverse correlation has not been extensively investigated adults. The PCV is now approved for use in adults, a group more likely to be colonized with S. aureus. To investigate the impact of PCV vaccination on S. aureus carriage in adults, the clinical evaluation of a multiplex Real Time PCR method to simultaneously detect very low levels of S. pneumoniae and S. aureus from the same nasal sample is described.
"]},{"key":"dc:title","label":"Title","values":["Evaluation of a Multiplex Real-Time PCR Assay to Simultaneously Detect Streptococcus pneumoniae and Staphylococcus aureus in Nasal Samples"]}]}],"canonical_facts":{"dc:contributor":["Paul Mann"],"dc:creator":["Hockberger, Polly"],"dc:date.available":["2016-12-03T08:00:00Z"],"dc:description.abstract":["Invasive disease caused by Streptococcus pneumoniae and Staphylococcus aureus is associated with nasal carriage of these bacteria. Use of a pneumococcal conjugate vaccine (PCV) in children altered nasal carriage rates and reduced infections due to S. pneumonia. Similar studies demonstrate that there is an inverse correlation between S. pneumoniae and S. aureus nasal carriage in children, but this inverse correlation has not been extensively investigated adults. The PCV is now approved for use in adults, a group more likely to be colonized with S. aureus. To investigate the impact of PCV vaccination on S. aureus carriage in adults, the clinical evaluation of a multiplex Real Time PCR method to simultaneously detect very low levels of S. pneumoniae and S. aureus from the same nasal sample is described.
"],"dc:identifier":["https://commons.nmu.edu/theses/117"],"dc:subject":["Multiplex","Real-Time PCR","Streptococcus pneumoniae","Staphylococcus aureus","Nasal","Other Analytical, Diagnostic and Therapeutic Techniques and Equipment"],"dc:title":["Evaluation of a Multiplex Real-Time PCR Assay to Simultaneously Detect Streptococcus pneumoniae and Staphylococcus aureus in Nasal Samples"],"thesis:degree_discipline":["Clinical Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T03:23:47Z"}