An in vitro study to characterize a new automated high throughput neuronal stretch injury system

Traumatic brain injury (TBI) is one of the most prevalent long-term neurological conditions and its overall economic consequences rival that of stroke. Considering the importance of the topic, new models for studying TBI are often designed and created. Based on a model developed by the University of Pennsylvania, NJIT constructed a fully automated version, which injures neuronal cell cultures in uniaxial regime and provides the high experimental yield required in pharmaceutical and neuroscience studies. In this investigation, a new methodology for culturing primary cortical neurons of rat origin was established. Viable cell cultures developed for the first time in NJIT laboratory were successfully injured using the new NJIT device. The data recorded here may allow us to understand better the complex aspects of head trauma mechanisms and to develop potential therapeutic agents.

Name thesis:degree_name

Master of Science in Biomedical Engineering - (M.S.)

Discipline thesis:degree_discipline

Biomedical Engineering

Year

2009

Author dc:creator

• Hususan, Nicolae Valerian

Contributors dc:contributor

• Bryan J. Pfister
• Richard A. Foulds
• Max Roman

• Traumatic brain injury
• Primary cortical neuron culturing
• Biomedical Engineering and Bioengineering

Repository record dc:identifier

https://digitalcommons.njit.edu/theses/308

OAI identifier oai:identifier

oai:digitalcommons.njit.edu:theses-1307