Protein responsive synthetic materials for biomedical applications

<p>"The production of synthetic materials with molecular recognition abilities towards biomacromolecules has been widely researched because of its potential applications in separations, sensing, therapeutics, etc. While molecular imprinting has become a commercially viable method for the production of synthetic materials with molecular recognition abilities towards small molecules, little success has been recorded towards protein targets. Proteins are difficult to imprint as they present a number of epitopes for imprinting, are difficult to remove from the completed material, and are incompatible with the densely crosslinked structures required. As a result, a novel surface molecular imprinting technique was developed to synthesize polymeric materials with molecular recognition abilities towards proteins. The recognitive surface of these materials was formed through the use of a templating mask where the target macromolecules, immunoglobulin G (IgG) and protein A in these studies, were immobilized. As a result, the surfaces of the molecularly imprinted materials were chemically and sterically complementary to the epitopes presented by the template molecules. The molecularly imprinted polymer (MIP) materials formed by this surface imprinting technique were able to recognize and rebind the template molecule, even when competing with structural analogs. This newly developed surface imprinting technique demonstrates potential to become a substitute for ELISA (Enzyme-Linked Immunosorbent Assay) or as a sensing element in microdevices formed through standard photolithographic processes"--Abstract, page iv.</p>

Name thesis:degree_name

Ph. D. in Chemical Engineering

Grantor

Missouri University of Science and Technology

Year dc:date.available

2016

Author dc:creator

• Zheng, Youyou

• Chemical Engineering

Repository record dc:identifier

https://scholarsmine.mst.edu/doctoral_dissertations/66

OAI identifier oai:identifier

oai:scholarsmine.mst.edu:doctoral_dissertations-1068