Selection for Run1-Ren1 Dihybrid Grapevines Using Microsatellite Markers

Powdery mildew is a major fungal disease of grapevine worldwide, and the control of which relies on large quantities of fungicide, accounting for a considerable cost for grape growers. Genetically controlled resistance is a promising alternative to the recurrent application of chemicals.The inheritance of Ren1 and Run1 powdery mildew resistance (R) genes were tracked in a grapevine hybrid family using flanking microsatellite markers. Segregation of the powdery mildew resistance phenotype was evaluated under in vitro and greenhouse conditions independently of the genotype data. Combined analysis of phenotypic and genotypic segregations confirmed that Ren1 and Run1 acted as single dominant loci, and assorted independently without considerable distortion of segregation. Recombinant chromosomes were detected in the Ren1 but not in the Run1 region, corroborating earlier observations that crossover events between homologous chromosomes are suppressed around the Run1 locus.Taken together, the results of this work show that microsatellite marker-assisted selection is a reliable and cost-effective method to combine multiple alleles that confer resistance to a pathogen.In addition, this work identified the progeny that inherited two R genes, which will be further crossed with a third powdery mildew R genes to develop a new table grape cultivar.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

2012

Author dc:creator

• Li, Chen

Contributors dc:contributor

• Laszlo Kovacs

• marker-assisted selection
• gene pyramiding
• Erysiphe necator
• Ren1
• Run1
• microsatellite
• Biology

Statement dc:rights

• © Chen Li

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1300

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2301