Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna

Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

1986

Author dc:creator

• Entrup, Mark Henry

Contributors dc:contributor

• Christopher Field

• Biology

Statement dc:rights

• © Mark Henry Entrup

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/70

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-1071