{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1071"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1071","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna","abstract":"Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed.","abstract_html":"Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed.","abstract_has_math":false,"creators":["Entrup, Mark Henry"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Christopher Field"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986-07-01T07:00:00Z","date_published":"1986-07-01T07:00:00Z","updated_at":"2026-07-24T03:14:59Z","subjects":["Biology"],"languages":[],"rights":["© Mark Henry Entrup"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/70","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Christopher Field"]},{"key":"dc:creator","label":"Author","values":["Entrup, Mark Henry"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Mark Henry Entrup"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/70"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed."]},{"key":"dc:title","label":"Title","values":["Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna"]}]}],"canonical_facts":{"dc:contributor":["Christopher Field"],"dc:creator":["Entrup, Mark Henry"],"dc:description.abstract":["Bacteriophage PBL1 was isolated from Bacillus larvae, a pathogen of the common honeybee. In this study, methods of propagation and concentration of PBL1 bacteriophage were developed. The DNA was extracted from the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double digestion, the order of the fragments were deduced and a physical map was developed."],"dc:identifier":["https://bearworks.missouristate.edu/theses/70"],"dc:rights":["© Mark Henry Entrup"],"dc:subject":["Biology"],"dc:title":["Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:14:59Z"}