Steroid Stimulated Protein Synthesis in Fish Cell Cultures

Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

1986

Author dc:creator

• Green, Carla B.

Contributors dc:contributor

• Christopher Field

• Biology

Statement dc:rights

• © Carla B Green

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/69

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-1070