{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1070"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1070","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Steroid Stimulated Protein Synthesis in Fish Cell Cultures","abstract":"Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography.","abstract_html":"Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography.","abstract_has_math":false,"creators":["Green, Carla B."],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Christopher Field"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986-05-01T07:00:00Z","date_published":"1986-05-01T07:00:00Z","updated_at":"2026-07-24T03:14:59Z","subjects":["Biology"],"languages":[],"rights":["© Carla B Green"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/69","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Christopher Field"]},{"key":"dc:creator","label":"Author","values":["Green, Carla B."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Carla B Green"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/69"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography."]},{"key":"dc:title","label":"Title","values":["Steroid Stimulated Protein Synthesis in Fish Cell Cultures"]}]}],"canonical_facts":{"dc:contributor":["Christopher Field"],"dc:creator":["Green, Carla B."],"dc:description.abstract":["Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for one or three hours. Testosterone produced the greatest response in CHSE-214 cells while progesterone and estrogen stimulated protein synthesis to a lesser degree. Gonad cells showed the greatest increase in protein synthesis when treated with progesterone and estrogen and showed only a minimal increase when treated with testosterone. Although steroid treatment influenced protein synthesis, it did not induce any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography."],"dc:identifier":["https://bearworks.missouristate.edu/theses/69"],"dc:rights":["© Carla B Green"],"dc:subject":["Biology"],"dc:title":["Steroid Stimulated Protein Synthesis in Fish Cell Cultures"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:14:59Z"}