Bacteriophage Sp10 Infection of Bacillus Subtilis

Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed in single-burst growth experiments and protein profiles were generated with PAGE-SDS. Analysis of the profiles in tube and/or slab gels revealed altered banding patterns with phage-infected versus uninfected cells. While no viral-coded nonstructural proteins were detected with this technique, five bands were assigned as viral structural proteins as evident in phage-infected cells.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

1985

Author dc:creator

• Katt, Wayne Charles

Contributors dc:contributor

• Christopher Field

• Biology

Statement dc:rights

• © Wayne Charles Katt

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/67

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-1068