{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1068"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1068","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Bacteriophage Sp10 Infection of Bacillus Subtilis","abstract":"Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed in single-burst growth experiments and protein profiles were generated with PAGE-SDS. Analysis of the profiles in tube and/or slab gels revealed altered banding patterns with phage-infected versus uninfected cells. While no viral-coded nonstructural proteins were detected with this technique, five bands were assigned as viral structural proteins as evident in phage-infected cells.","abstract_html":"Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed in single-burst growth experiments and protein profiles were generated with PAGE-SDS. Analysis of the profiles in tube and/or slab gels revealed altered banding patterns with phage-infected versus uninfected cells. While no viral-coded nonstructural proteins were detected with this technique, five bands were assigned as viral structural proteins as evident in phage-infected cells.","abstract_has_math":false,"creators":["Katt, Wayne Charles"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Christopher Field"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1985,"date_issued":"1985-05-01T07:00:00Z","date_published":"1985-05-01T07:00:00Z","updated_at":"2026-07-24T03:14:59Z","subjects":["Biology"],"languages":[],"rights":["© Wayne Charles Katt"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/67","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Christopher Field"]},{"key":"dc:creator","label":"Author","values":["Katt, Wayne Charles"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Wayne Charles Katt"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/67"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed in single-burst growth experiments and protein profiles were generated with PAGE-SDS. Analysis of the profiles in tube and/or slab gels revealed altered banding patterns with phage-infected versus uninfected cells. While no viral-coded nonstructural proteins were detected with this technique, five bands were assigned as viral structural proteins as evident in phage-infected cells."]},{"key":"dc:title","label":"Title","values":["Bacteriophage Sp10 Infection of Bacillus Subtilis"]}]}],"canonical_facts":{"dc:contributor":["Christopher Field"],"dc:creator":["Katt, Wayne Charles"],"dc:description.abstract":["Initial phenotypic characterization, methods of phage propagation, and complementation studies were performed with phage SP10 and SP10c, a clear plaque mutant derived from SP10. A propagation scheme was developed to generate high titer lysates of Bacillus subtilis W23. Phage production was analyzed in single-burst growth experiments and protein profiles were generated with PAGE-SDS. Analysis of the profiles in tube and/or slab gels revealed altered banding patterns with phage-infected versus uninfected cells. While no viral-coded nonstructural proteins were detected with this technique, five bands were assigned as viral structural proteins as evident in phage-infected cells."],"dc:identifier":["https://bearworks.missouristate.edu/theses/67"],"dc:rights":["© Wayne Charles Katt"],"dc:subject":["Biology"],"dc:title":["Bacteriophage Sp10 Infection of Bacillus Subtilis"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:14:59Z"}