Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus

Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

1986

Author dc:creator

• Stewart, Annette Renee

Contributors dc:contributor

• Christopher Field

• Biology

Statement dc:rights

• © Annette Renee Stewart

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/65

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-1066