{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1066"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1066","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus","abstract":"Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues.","abstract_html":"Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues.","abstract_has_math":false,"creators":["Stewart, Annette Renee"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Christopher Field"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986-07-01T07:00:00Z","date_published":"1986-07-01T07:00:00Z","updated_at":"2026-07-24T03:14:59Z","subjects":["Biology"],"languages":[],"rights":["© Annette Renee Stewart"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/65","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Christopher Field"]},{"key":"dc:creator","label":"Author","values":["Stewart, Annette Renee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Annette Renee Stewart"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/65"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues."]},{"key":"dc:title","label":"Title","values":["Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus"]}]}],"canonical_facts":{"dc:contributor":["Christopher Field"],"dc:creator":["Stewart, Annette Renee"],"dc:description.abstract":["Infectious Pancreatic Necrosis (IPN) virus is an economically important pathogen of young, hatchery-reared trout. Little information is available on the early steps of IPN virus infection. In this study a method of assaying attachment of IPN viruses to Chinook Salmon Embryo (CHSE) cells was developed. The attachment of ³⁵S-methionine-labeled viruses was assayed over time and at various temperatures. IPN virus receptors on CHSE cells were treated with trypsin, chymotrypsin, neuraminidase, protease, and concanavalin A (Con A) and the subsequent attachment of ³⁵S-methionine-labeled viruses assayed. IPN virus required 10 seconds to attach to CHSE cells. Significantly more viruses were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues."],"dc:identifier":["https://bearworks.missouristate.edu/theses/65"],"dc:rights":["© Annette Renee Stewart"],"dc:subject":["Biology"],"dc:title":["Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:14:59Z"}