Searching for M13 bacteriophage with high affinity for nanodiamond particles

Nanodiamonds have potential in biomedical uses, as they are non-toxic and exhibit non-blinking fluorescence behavior when they are enriched with nitrogen vacancy centers. In order for them to be useful in biomedical applications, they need to be functionalized. In this experiment, a pIII library of M13 bacteriophage were panned versus 100 nm nanodiamonds enriched with approximately 500 nitrogen vacancy centers to find phage that have an affinity for these nanoparticles. The phage DNA was sequenced and found to have the protein sequence SKMYHTP. At this point, although we have sequences of peptide that bind to nanodiamond, we are unable to determine the affinity or best binders without additional biopanning rounds and testing.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Materials Science and Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2013

Author dc:creator

• Au, Ho Yin

Advisor dc:contributor.advisor

• Angela Belcher.

• Materials Science and Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/81138

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/81138