{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/81138"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/81138","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Searching for M13 bacteriophage with high affinity for nanodiamond particles","abstract":"Nanodiamonds have potential in biomedical uses, as they are non-toxic and exhibit non-blinking fluorescence behavior when they are enriched with nitrogen vacancy centers. In order for them to be useful in biomedical applications, they need to be functionalized. In this experiment, a pIII library of M13 bacteriophage were panned versus 100 nm nanodiamonds enriched with approximately 500 nitrogen vacancy centers to find phage that have an affinity for these nanoparticles. The phage DNA was sequenced and found to have the protein sequence SKMYHTP. At this point, although we have sequences of peptide that bind to nanodiamond, we are unable to determine the affinity or best binders without additional biopanning rounds and testing.","abstract_html":"Nanodiamonds have potential in biomedical uses, as they are non-toxic and exhibit non-blinking fluorescence behavior when they are enriched with nitrogen vacancy centers. In order for them to be useful in biomedical applications, they need to be functionalized. In this experiment, a pIII library of M13 bacteriophage were panned versus 100 nm nanodiamonds enriched with approximately 500 nitrogen vacancy centers to find phage that have an affinity for these nanoparticles. The phage DNA was sequenced and found to have the protein sequence SKMYHTP. At this point, although we have sequences of peptide that bind to nanodiamond, we are unable to determine the affinity or best binders without additional biopanning rounds and testing.","abstract_has_math":false,"creators":["Au, Ho Yin"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Materials Science and Engineering.","school":null,"contributors":[],"advisors":["Angela Belcher."],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013","date_published":"2013","updated_at":"2026-07-22T22:21:20Z","subjects":["Materials Science and Engineering."],"languages":["eng"],"rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 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In order for them to be useful in biomedical applications, they need to be functionalized. In this experiment, a pIII library of M13 bacteriophage were panned versus 100 nm nanodiamonds enriched with approximately 500 nitrogen vacancy centers to find phage that have an affinity for these nanoparticles. The phage DNA was sequenced and found to have the protein sequence SKMYHTP. At this point, although we have sequences of peptide that bind to nanodiamond, we are unable to determine the affinity or best binders without additional biopanning rounds and testing."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.B."]},{"key":"dc:title","label":"Title","values":["Searching for M13 bacteriophage with high affinity for nanodiamond particles"]}]}],"canonical_facts":{"dc:contributor.advisor":["Angela Belcher."],"dc:contributor.department":["Massachusetts Institute of Technology. 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In this experiment, a pIII library of M13 bacteriophage were panned versus 100 nm nanodiamonds enriched with approximately 500 nitrogen vacancy centers to find phage that have an affinity for these nanoparticles. The phage DNA was sequenced and found to have the protein sequence SKMYHTP. At this point, although we have sequences of peptide that bind to nanodiamond, we are unable to determine the affinity or best binders without additional biopanning rounds and testing."],"dc:description.degree":["S.B."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/81138"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 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