Comparison of phenanthriplatin, a novel monofunctional platinum based anticancer drug candidate, with cisplatin, a classic bifunctional anticancer drug

Nucleotide excision repair, a DNA repair mechanism, is the major repair pathway responsible for removal of platinum-based anticancer drugs. In this study, 146 bp duplexes were prepared containing either a site-specific cisdiammineplatinum( Il)-DNA intrastrand d(GpG) cross-link or a cisdiamminephenanthridinechloroplatinum( Il)-DNA dG adduct. Comparison of the repair efficiencies of the two adducts reveals that the diamminephenanthridinechloroplatinum(lI)-DNA dG lesion eludes the nucleotide excision repair pathway better than diammineplatinum(lI)-DNA intrastrand d(GpG) cross-link. A factor that may be relevant to the difference is the influence of platination on DNA-mediated charge transfer. Atomic force microscopy is a method by which we can explore the possibility that phenanthriplatin influences charge transfer properties of DNA. Long DNA duplexes site-specifically modified with cisplatin or phenthanriplatin were prepared for AFM studies.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemistry.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2012

Author dc:creator

• Li, Meiyi, S.M. Massachusetts Institute of Technology

Advisor dc:contributor.advisor

• Stephen J. Lippard.

• Chemistry.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/78439

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/78439