E2F6 in axial skeletal development and gliosis

E2F transcription factors were originally identified as regulators of cell cycle and cellular proliferation. In vivo mouse models have uncovered novel roles for these proteins in different developmental processes. This dissertation examines the biological role of E2F6 in mammalian development. E2F6 functions as a repressor of transcription in concert with the polycomb group (PcG) proteins and chromatin modifiers. PcG proteins regulate processes required for proper embryonic development and differentiation. E2F6 interacts with core components of Polycomb Repressive Complex 1 (PRC1) and participates in PcG-mediated repression of Hox genes. Hox genes are required for correct patterning of the mammalian skeleton. Loss of E2f6 results in posterior axial skeletal transformations. Mice deficient for both E2f6 and Bmil, a component of PRC1, exhibit increased penetrance of axial skeletal transformations. Thus, E2F6 and Bmil cooperate in the regulation of Hox genes and axial skeletal development. Bmi 1 also represses transcription of the Ink4a-Arf locus, and it is consequently required to maintain the proliferative and self-renewal properties of hematopoietic and neural stem cells. However, E2F6 does not participate in the repression of the Ink4a-Arflocus. These findings underscore the significance of the E2F6-Bmil interaction in vivo and suggest that the Hox and Ink4a-Arfloci are regulated by somewhat different mechanisms. In addition to axial skeletal transformations, E2f6' mice exhibit a suppressed gliotic response after neural injury. Gliosis occurs in response to neurodegeneration, ischemia, and neuronal cell death. This process provides neuronal protection by restricting inflammation and regulating the concentration of molecules in the extracellular environment.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Biology.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2008

Author dc:creator

• Friesenhahn, Laurie Beth

Advisor dc:contributor.advisor

• Jacqueline A. Lees.

• Biology.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/45802

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/45802