Roles for motifs of cell cycle regulating kinases beyond substrate selection of individual kinases

Errors in the cell cycle, particularly during mitosis, have recently been implicated in tumorigenesis and cancer formation. Several protein kinases, including the major mitotic kinases Cdkl, Aurora A, Aurora B, Nek2, and Plkl, and the Polo-like kinase (Plk) family, Plkl, Plk2, Plk3, and Plk4, are known to play important roles in the regulation of mitosis and other phases of the cell cycle to prevent such errors. However, the mechanisms underlying many of the roles of these kinases are unknown because the appropriate substrates have not been identified. To aid in substrate identification and to gain insight into the role of substrate specificity in the regulation of these kinases, we have determined the motifs of the major mitotic kinases and the Plk family of kinases by Positional Scanning Oriented Peptide Library Screening (PS-OPLS), which gives complete, unbiased motifs. The PS-OPLS motif of Plkl revealed a previously unreported specificity of Plkl leading to the identification of new candidate substrates, including p31/Comet, which is involved in the silencing of the spindle assembly checkpoint for anaphase onset. Additionally, the motifs and the localizations of the major mitotic kinases lead us to put forward a model potentially explaining how the major mitotic kinases, despite having overlapping localizations and access to the same sites on substrates, phosphorylate distinct sites. This model, if true, would suggest that the motifs and localizations of all of the major mitotic kinases coordinately direct the group of kinases to the appropriate substrates. The motifs of the Plk family reveal that Plk2 and Plk3 phosphorylate sites previously primed by phosphorylation by a tyrosine kinase, suggesting that these kinases may integrate tyrosine kinase and serine/threonine kinase signaling in a novel way.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Biology.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2008

Author dc:creator

• Alexander, Jes

Advisor dc:contributor.advisor

• Michael B. Yaffe.

• Biology.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/45313

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/45313