Inhibition of Hsp90 in Streptomyces coelicolor

Inhibition of the chaperone protein Hsp90 in plants and insects has been found to result in drastic changes in phenotype. We investigated the effect of Hsp90 inhibition on the bacteria Streptomyces coelicolor. These changes were studied by growing S. coelicolor in the presence of Hsp90 inhibitors, extracting the growth media, and subjecting the extractions to HPLC and MS analysis. It has been found that reproducibility is difficult to achieve. Since the genome of S. coelicolor has been sequenced, attention was turned to studying changes in mRNA expression levels, which gave more reproducible results, as well as insight to the specific genes being affected.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Chemistry.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Wu, Katherine A. (Katherine Ann)

Advisor dc:contributor.advisor

• Sarah E. O'Connor.

• Chemistry.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/36280

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/36280