Integrated microbioreactors for rapid screening and analysis of bioprocesses

(cont.) strains to characterize the bioreactor environment. The ability to reproducibly sacrifice microbioreactors mid-run is exploited to demonstrate the feasibility of linking microbioreactors to genome-wide expression studies using DNA microarrays. The potential of the microbioreactor for investigating different growth conditions is confirmed by comparing bacterial growth, as evaluated by the measured parameters, under conditions of different medium and oxygen concentration. It is shown that statistical differences can be observed, and that these differences are similar to those observed at a larger scale. The demonstrated functionality of the microbioreactor could potentially have a large impact in the numerous fields in which fermentations are used. In bioprocess development, the batch microbioreactor could be used to select strains at all stages of metabolic engineering and to explore and optimize growth conditions during scale-up. The microbioreactor could also be an effective tool in screening applications ranging from toxicology studies that use bacterial reporter strains, to studies that attempt to elucidate metabolic pathways, to intensification of genome-wide expression profiling using either direct links to DNA microarrays or screens of libraries carrying transcription reporters.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Chemical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Zanzotto, Andrea, 1975-

Advisor dc:contributor.advisor

• Klavs F. Jensen.

• Chemical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/28844

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/28844