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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 72 for “"DNA microarrays"”.
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Photoelectromechanical synthesis of low-cost DNA microarrays
… genomic selection for target sequencing using DNA from custom microarrays have demonstrated that microarrays can effectively be used as the world's cheapest sources of complex oligonucleotide pools. Unfortunately, commercial custom microarrays are expensive and not easily accessible to academic …
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Analyse des Hitzeschocks bei Neisseria meningitidis mit DNA-Microarrays
… In dieser Arbeit wurde die neue Technologie der DNA Mikroarray Technologie für die Untersuchung des Transkriptoms bei Neisseria meningitidis etabliert. Untersucht wurde die Reaktion von N. meningitidis auf einen Hitzeschock, eine plötzliche Steigerung der Temperatur. Während einer Infektion wird …
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Typing and genome comparison of Neisseria meningitidis by DNA-microarrays
… have been followed. The first one evaluated microarrays based on polymorphism-directed oligonucleotide design for typing of N. meningitidis adopting the multilocus sequence typing (MLST) concept. The number of oligonucleotides needed to cover all known polymorphisms was much lower compared to …
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Analyse des Transkriptoms von Neisseria meningitidis Serogruppe B mit DNA-Microarrays
… Serogruppe B wurde im Vergleich mit einem cDNA-basierten System demonstriert. Hierzu wurde für ein Subset von 60 Genen die Eigenschaften anhand von Parallelhybridisierungen sowie eines vormals etablierten Hitzeschock Modells untersucht. Aufgrund der Vergleichsdaten wurde ein …
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Fabrication of DNA microarrays on Poly (methylmethacrylate) substrates for biomolecular reporting
DNA microarrays require the use of substrates with well-established surface modification / probe attachment chemistries. Glass/quartz have been widely adopted as typical support materials since their surface modification chemistries which involve the use of siloxane –based chemistries have been …
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Studien der Genexpressionsänderung während der Entwicklung von Drosophila melanogaster mittels DNA-Microarrays
… identifizieren wurde im Rahmen dieser Arbeit ein DNA-Microarray entwickelt, mit dem Veränderungen in der mRNA-Expression analysiert wurden. Auf der Grundlage einer neuentwickelten ab initio Genvorhersage im Rahmen einer Koorporation wurden spezifische Primer generiert, die eine Amplifikation der …
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Entwicklung eines DNA Microarrays zur genomweiten Analyse Polycomb-vermittelter Genregulation in Drosophila melanogaster
… solche Fragestellungen ausgerichtet ist, nutzt DNA Microarrays. Mit ihrer Hilfe kann die Expression von Genen eines gesamten Genoms zu bestimmten Zeitpunkten oder in verschiedenen Geweben untersucht werden. Auch epigenetische Mechanismen der Regulation können mit einem solchen System untersucht …
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Real-time Three-Dimensional DNA Microarrays: How Well Can We Distinguish Between Related Target Sequences?
DNA microarrays, due to their highly parallel nature, are in principle well suited for rapid identification of known or related microbial species, but our ability to extract meaningful information from microarray images is still at a rudimentary level. The use of DNA microarrays is currently …
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Expression Profiling by DNA Microarrays : Development of Amplification Methods for the Analysis of Minimal Tumor Samples
Recently, microarrays of synthetic long sense-oriented oligonucleotides were introduced as an alternative expression profiling platform with distinct advantages to both cDNA arrays and commercial arrays produced by in situ synthesis of multiple short oligonucleotides per gene. However, gene …
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Design, manufacture, and application of DNA microarrays to study gene expression phenotypes of lysine-producing Corynebacterium glutamicum
Corynebacterium glutamicum partial genome DNA microarrays were constructed that were capable of assaying the transcriptional profile of the genes of pathways involved in central carbon metabolism and lysine biosynthesis. It was found that to ensure arrays of high quality, protocols applying the …
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An analysis of the Supramolecular NanoStamping technology for its market potential based upon a review of DNA microarray intellectual property
… fabrication. The technology may be used to make DNA microarrays which are an essential tool to genomic research assisting in gene expression and genotyping. This paper explores the potential of bring Supramolecular NanoStamping technology to the microarray market. An in depth analysis of the …
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Integrated characterization of cellular physiology underlying hepatic metabolism
… in the environment. The data provided by DNA microarrays and multiple types of metabolic measurements can be integrated to reconstruct the actions taken by a cellular system to arrive at a particular metabolic response to a stimulus, elucidating the underlying physiology. We employed this …
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Integrated microbioreactors for rapid screening and analysis of bioprocesses
… to genome-wide expression studies using DNA microarrays. The potential of the microbioreactor for investigating different growth conditions is confirmed by comparing bacterial growth, as evaluated by the measured parameters, under conditions of different medium and oxygen concentration. …
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Genomic analysis of hepatic insulin resistance
… glycemia. To complement these efforts, we used DNA microarrays to find differentially expressed genes and combinatorial siRNA screening to investigate the effects of hepatic gene transcription during periods of high and low glucose production. This strategy provides a new approach to studying …
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DNA hybridization : fundamental studies and applications in directed assembly
Programmed self-assembly using non-covalent DNA-DNA interactions is a promising technique for the creation of next-generation functional devices for electronic, optical, and magnetic applications. This thesis develops the ability to tailor surfaces for the DNA-driven assembly of molecular, nano-, …
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A Comparison of Microarray Analyses: A Mixed Models Approach Versus the Significance Analysis of Microarrays
DNA microarrays are a relatively new technology for assessing the expression levels of thousands of genes simultaneously. Researchers hope to find genes that are differentially expressed by hybridizing cDNA from known treatment sources with various genes spotted on the microarrays. The large number …
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Global transcriptional analysis of an Escherichia coli recombinant protein process during hypoxia and hyperoxia
… This work demonstrates the utility of DNA microarrays in analyzing and improving microbial fermentations. Global expression studies have suggested several strategies for increasing the resistance of bioprocesses to the damaging effects of oxygen and recombinant protein production.
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Photoelectrochemical array platform for genomic scale DNA synthesis
… for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled into large constructs of DNA for costs significantly less than traditional methods. This thesis presents a complete platform for the …
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Biologically-Interpretable Disease Classification Based on Gene Expression Data
… expression data is a powerful application of DNA microarrays. Many popular classifiers like support vector machines, nearest-neighbour methods, and boosting have been applied successfully to this problem. However, it is difficult to determine from these classifiers which genes are responsible …
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Supramolecular nano-stamping : analyzing market potential
… the science involved has market potential in the DNA microarray industry. This industry is rapidly expanding and succeeding in such an atmosphere would generate a large amount of revenue. Supramolecular Nano-Stamping provides a way to reproduce DNA microarrays at a significantly lower cost than …
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