Characterization of two Bacillus subtilis proteins required for the initiation, restart, and control of DNA replication

(cont.) This is consistent with an inability of dnaBS371P cells to adjust the frequency of initiation according to growth rate. I also found that cells over-producing DnaBS371P are filamentous, contain decreased DNA contents, and are hypersensitive to DNA-damaging agents. These abnormalities may result from a defect in replication restart at damaged or stalled replication forks. Thus, whereas dnaBS3 71P suppresses the defects of mutant cells that cannot initiate or restart replication, expressing DnaBS371P in wild-type cells causes defects in initiation and restart.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biology

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2004

Author dc:creator

• Rokop, Megan E., 1978-

Advisor dc:contributor.advisor

• Alan D. Grossman.

• Biology.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/28673

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/28673