On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves

Recent proteomics researches require a sensitive, high-speed, and automatic protein separation tool that far exceeds the capability of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve control in a microchip. Several experiments demonstrating independent 2D separation were performed, and critical parameters for better chip performance were identified; including channel passivation, electroosmosis control, IEF linearity control, and detection enhancement. The result can be used for the filtration of high-abundance proteins, which used to be done by affinity columns. Also, it can be used for much possible integration between different heterogeneous separation/analysis techniques such as IEF, polyacrylamide gel electrophoresis (PAGE), CE, reverse-phase chromatography, and mass spectrometry (MS).

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2004

Author dc:creator

• Wang, Ying-Chih, 1977-

Advisor dc:contributor.advisor

• Jongyoon Han.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/27143

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/27143