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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Protein separation"”.
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Hydrocyclone fractionation of chickpea flour and measurement of physical and functional properties of flour and starch and protein fractions
… contains a high amount of starch and valuable protein. Many grain legumes (pulses) can be processed by pin milling and air classification with high separation efficiency. However, chickpea exhibits low separation efficiency because it has a relatively high fat content compared to other pulses. …
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Ultrasound-assisted enzymatic extraction of protein hydrolysates from brewer's spent grain
… cattle feed. Since BSG contains 20 to 25% of proteins, it has the potential to provide a new protein source to the food industry. In this research, an ultrasound-assisted enzymatic extraction was designed to extract protein hydrolysates from BSG. Original BSG and ultrasound pretreated BSG were …
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CE-microreactor-CE-MS-MS for protein analysis
… handling and allows fast digestions of small protein samples.In this thesis work, an on-line CE-microreactor-CE-MS-MS instrument is developed that can separate proteins in a first dimension, digest them inside a pepsin-immobilized microreactor, separate peptides in a second dimension, and …
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Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration
… optimize the efficiency of monoclonal antibody separation and purification for an industrial process. A base case was established that utilized common protein separation techniques such as cation exchange chromatography, anion exchange chromatography, and hydrophobic interaction chromatography …
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On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves
… require a sensitive, high-speed, and automatic protein separation tool that far exceeds the capability of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple …
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Proteomic Profiling of Animal Models of Motor Neuron Disease and Muscular Dystrophy
… and/or liquid chromatography for largeQscale protein separation, and labelQfree mass spectrometry basedQproteomics are highly suitable to determine changes in the isoform expression pattern of muscle and testis proteins. The work presented outlines major categories of protein families that …
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Multidimensional liquid chromatographic separations for proteomics
… to reduce this complexity, both at an intact protein and digested peptide level. For complex lysates containing many thousands of proteins, orthogonal (mutually independent), multidimensional separation methods must be employed to provide sufficient resolution to characterize the appropriate …
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Proteomics approach to investigate separation behavior of proteins in ion-exchange chromatography
<p>In the present study, protein isoelectric point (pI), molecular weight (MW) and partitioning coefficient (K) were characterized for a mixture of proteins using a three-dimensional (3D) method which combined hydrophobic partitioning with two-dimensional (2D) electrophoresis. Protein pI and MW …
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Protein LC-MS Using Slip Flow Chromatography
<p>Histones are essential chromosomal proteins with large numbers of variants and post-translational modifications (PTMs). PTM levels of histones are known to correlate with different stages of cancer. Due to the lack of resolution on intact histone separations, conventional methods for histone …
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Designing Magnetically Responsive Ultrafiltration Membranes
… (UF) membranes developed out of a need for protein separation processes. Currently, they are used in a variety of industries ranging from food manufacturing to pharmaceuticals for two main purposes: concentration, separation, and buffer exchange. UF membrane processes in product streams …
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Proteomic Analysis of Plasma Membrane Proteins from Drug Susceptible and Drug Resistant Breast Cancer Cell Lines
… membranes are rich in drug targets and other proteins responsible for cell signaling, transport, signal transduction, and other cellular functions. Information obtained about these proteins, and the pathways they participate in, helps to facilitate the drug discovery process. Although these …
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Countercurrent chromatography of proteins using aqueous two-phase systems
… biotechnology industry requires high - capacity protein manufacturing processes that retain protein functionality. Large - scale countercurrent J- type centrifuges have been developed by the Brunel Institute for Bioengineering that successfully purify small organic molecules using aqueous - …
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CMOS nanofluidics
… An important part of clinical diagnosis involves protein and DNA sensing. Significant effort is made to make protein and DNA sensing more accessible and affordable, through micro and nano-technologies. However, typical commercial and academic devices for molecular sensing suffered needs for …
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Multidimensional Protein Separations in a Plastic Microfluidic Network
… for performing reversed-phase peptide separations. Separation performance of cytochrome C digest in miniaturized membrane chromatography is compared with the results obtained from μ-LC and capillary LC. The use of miniaturized membrane chromatography allows significant reduction in …
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Development and Testing of a Rapid Multiplex Assay for the Identification of Biological Stains
… Identification – Utilizing multidimensional protein separation technologies, bioinformatics tool, and tandem mass spectrometry a database of fluid-specific candidate markers will be developed for each body fluid.</p> <p>2. Biomarker Verification – Using the most promising candidates from …
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Effects of Charge Distribution on Protein Solution Properties
… the electrostatic properties of a series of proteins are characterized experimentally and related to theoretical predictions. The strength of protein interactions is characterized as a function of solute concentration, pH, and ionic strength. The strength of interaction and the solubility of …
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Microfluidic Tools for Advanced Biomolecular Characterisation
Proteins – the key building blocks of life – are responsible for the majority of the processes behind biological function. To understand what role proteins play in health and disease, how they operate and interact, it is vital to have tools for biomolecular detection, quantification and fundamental …
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Development of a novel liquid chromatography based tool to study post-translational modifications
… towards the individual modification and involves separation of modified proteins from non-modified ones. The drawback of using a modification specific method is that there is a lack of flexibility in its usage for other modifications. The goal of these studies was to investigate the possibility of …
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APPLICATIONS OF DYNAMIC ISOELECTRIC/ANISOTROPY BINDING LIGAND ASSAY FOR PROTEOMIC RESEARCH
… this work reviews the need for high efficiency protein separation techniques. Dynamic isoelectric focusing (DIEF) is new technique similar to capillary isoelectric focusing (CIEF) invented by Dr. Luke Tolley at Southern Illinois University Carbondale. Using DIEF, the electric field inside the …
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