Identification and characterization of rickettsial proteins at the host-pathogen interface

Bacterial pathogens subvert host cell processes during infection by secreting protein effectors that manipulate host machinery. Cataloging such effectors has enabled a deeper exploration of the molecular basis of disease for numerous pathogens. Members of the Rickettsia genus are obligate intracellular bacteria that pose a growing threat to human health, but their complete dependence on the host cell niche has precluded a thorough investigation of the bacterial factors acting at the host-pathogen interface. Accurately identifying and characterizing these proteins will provide a necessary framework for understanding rickettsial biology and disease. In this work, I demonstrate that the conserved rickettsial protein RARP-1 is not a bona fide secreted effector, as had been previously suggested. Instead, I found that Rickettsia parkeri RARP-1 localizes to the periplasm where it supports the rickettsial life cycle by promoting host cell invasion and intracellular growth. Motivated by this discrepancy, I developed a cell-selective proteomic screen to identify effectors secreted during R. parkeri infection. In addition to several known secreted effectors, my approach revealed the novel secreted rickettsial factors SrfA–G. Notably, these Srfs include Rickettsia-specific proteins of unknown function that are structurally diverse, variably conserved, and targeted to distinct host cell compartments. I further demonstrate that one of these effectors, SrfD, localizes to the endoplasmic reticulum where it interacts with the host Sec61 translocon. Taken together, this work highlights the elusive nature of rickettsial effectors while offering new ways to probe the unique biology of these bacterial pathogens.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biology

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2024

Author dc:creator

• Sanderlin, Allen Garrett

Advisor dc:contributor.advisor

• Lamason, Rebecca L.

Statement dc:rights

• In Copyright - Educational Use Permitted
• Copyright retained by author(s)

Licence dc:rights.uri

• https://rightsstatements.org/page/InC-EDU/1.0/

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/155408

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/155408