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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"Periplasm"”.
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Copper/zinc Sods of Salmonella Typhimurium: Enzymatic Properties and Correlation to Pathogenesis
… SodCI is a dimer and is not released from the periplasm by osmotic shock. Thus SodCI is ""tethered"" in the periplasm by some non-covalent interaction. In contrast, SodCII is monomeric and is quantitatively released by osmotic shock. Using site-directed mutagenesis we constructed a monomeric, …
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Antimicrobial Effectors Act Cooperatively to Stress Salmonella in the Macrophage Phagosome
… macrophages. The bacterium produces two Cu-Zn periplasmic superoxide dismutases, SodCI and SodCII. Although both enzymes are expressed during infection, only SodCI contributes to virulence in the mouse by combating phagocytic superoxide. We have shown that the differential contribution to …
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Investigating the key components of the chitinase secretion system in <i>Serratia marcescens</i>
… with initial export to the bacterial periplasm being followed by a final secretion step across the outer membrane. Successful secretion of the chitinolytic machinery ultimately depends on the products of a four-gene operon, <i>chiWXYZ</i>, which resembles a phage lysis cassette. At the …
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The development of a new reporter system to study the interaction between FbpA and FbpBC
… of iron from host transferrin and into the periplasm, where it binds to a periplasmic binding protein, ferric binding protein (FbpA). FbpA is part of the ABC transporter, FbpABC that transports iron from the periplasm into the cytoplasm. The interaction between FbpA and FbpBC is currently …
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Improving the recovery of "difficult to release" periplasmically-expressed products from recombinant E. Coli
The periplasm of E. coli has been recognized a suitable location for the production of large quantities of many industrially important soluble recombinant proteins, and offers some important advantages over both intracellular and extracellular production. The oxidative environment of the periplasm …
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Development of high throughput screening systems for the efficient production of antibody fragments in Escherichia coli
… tool. In the APEx 2-hybrid system, the bacterial periplasm constitutes the milieu for the association of membrane-anchored bait protein and solubly expressed, epitope-tagged prey protein. Upon disruption of the outer membrane, only prey proteins that bind to the bait remain cell-associated and are …
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Structural Insights into Modification and Export of the Pseudomonas aeruginosa Alginate Exopolysaccharide
… polymerization and translocation into the periplasm, the polymer can be modified by epimerization by AlgG, or acetylation by the concerted action of AlgI, AlgJ, AlgF, and AlgX. We proposed that AlgF is involved in protein-protein interactions mediating formation of an AlgJFX periplasmic …
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The Molecular Mechanism of the Escherichia Coli vitamin B12 Transporter BtuCD-F: Real-time Observation of the Transporter in Motion
… resulting in opening of the TMDs instead to the periplasm. However, the crystal structures of BtuCD from E. coli and recent EPR spectroscopy studies indicate that this type II importer employs a substantially different mechanism in which the TMDs are open to the periplasm in the resting state and …
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Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1
… implying" an" electron" storage" role" in" the" periplasm" during"respiration." "Finally,"the" structure" of" MtrCAB" was" investigated" through" small" angle" neutron" scattering" (SANS)."Structures"of"MtrCAB"were"highly"elongated"with"a"globular"head" region"and"a" tail" …
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Surface Localization Determinants of Borrelia burgdorferi Lipoproteins
… by epifluorescence microscopy in both the periplasm and on the bacterial surface. These findings supported the notion that Borrelia spirochetes do not adhere to the +2/+3/+4 sorting rules established in other eubacteria. Rather, borrelial lipoproteins seem to contain a disordered `tether' …
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Screen design for Borrelia burgdorferi lipoprotein secretion pathway components using periplasmic protein capture and suppressor mutations
… Binding Peptide and peptidoglycan) within the periplasm of B. burgdorferi, in an effort to stall the localization process. Based on our earlier work with Borrelia surface lipoproteins, a series of deletions within the intrinsically disordered N-terminal CspA tether were generated. Deletions of …
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Bacterial lipoproteins: sorting mechanisms and biotechnological applications
… associated to the outer membrane but facing the periplasm. To elucidate whether the difference in lipoprotein location is intrinsic to lipotrotein sequence/structure or rather is due to specific transport systems present in some bacterial species and absent in others lipoproteins were expressed …
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Superoxide Toxicity in Escherichia Coli
… little is understood about the physiology of the periplasm. of gram negative bacteria. The presence of CuZnSOD within that compartment in several different species suggests that oxidative damage occurs there. We studied the regulation of sodC, the gene that encodes CuZnSOD, and the phenotypes of …
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Secretion of the chitinolytic machinery in Serratia marcescens
… on the Sec or Tat machineries for export to the periplasm (two-step systems). Serratia marcescens is an important opportunistic human pathogen and has gathered a lot of interest due to its repertoire of secreted proteins. These include the haem-scavenging protein HasA, which is secreted by a Type …
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Investigation of CNT-induced Escherichia coli Lysis and Protein Release
… cell walls to make E. coli permeable, causing periplasm proteins and enzymes to leak out into the medium. Consequently, CNTs can be used as lysis agents when it is undesirable to add an additional enzyme (lysozyme) to cause the release of intracellular proteins.</p>
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Analysis of genes and enzymes involved in the degradation of cellulose and proteins by Butyrivibrio fibrisolvens H17c
… of the endoglucanase activity was located in the periplasm of the E. coli host. TnphoA mutagenesis indicated the presence of a functional E. coli-like signal peptide. The nucleotide sequence of end1 was determined and the amino acid sequence (547 amino acids) deduced. The catalytic domain of End1 …
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Synthesis of D-alanyl-D-alanine dipeptide isosteres and cephalosporin prodrugs
… as delivering the coupled antibiotic into the periplasm by enzymatic hydrolysis of the prodrug. A novel phase-transfer reaction was used to couple dipeptide isosteres to a cephalosporin intermediate.
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Outer Membrane Vesicle Production in Escherichia coli Relieves Envelope Stress and is Modulated by Changes in Peptidoglycan
… buds of the outer membrane (OM) containing periplasmic lumenal components. OMVs have been demonstrated to play a critical part in the transmission of virulence factors, immunologically active compounds, and bacterial survival, however vesiculation also appears to be a ubiquitous …
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Regulation of the periplasmic stress responses in E. coli and P. aeruginosa
… aeruginosa. These pathways sense stress in the periplasm and relay the signal into the cytoplasm by a series of proteolytic cleavages of a transmembrane regulatory protein. In E. coli, I have undertaken the study of the regulation of the cleavage of transmembrane regulator RseA by the first …
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Identification and characterization of rickettsial proteins at the host-pathogen interface
… that Rickettsia parkeri RARP-1 localizes to the periplasm where it supports the rickettsial life cycle by promoting host cell invasion and intracellular growth. Motivated by this discrepancy, I developed a cell-selective proteomic screen to identify effectors secreted during R. parkeri infection. …
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