Profiling hotspots of DNA breaks and learning-induced gene expression in the mouse brain

Neuronal activity generates DNA double-strand breaks (DSBs) in the brain. Topoisomerase IIb (TOP2B), important for relieving transcription-associated DNA supercoiling, was implicated as the source of these neuronal activity-induced DSBs, facilitating rapid transcriptional induction of immediate early genes (IEGs). However, the locations of these DSBs in vivo and their relation to brain function was unclear. In Chapter 2, following contextual fear conditioning (CFC) of wild type mice, we profiled the locations of DSBs genome-wide through gH2AX ChIP-Seq, along with transcriptomic changes in neuronal and glial-enriched nuclei in two brain regions. We found DSB-susceptible genes were involved in synaptic processes, while both activity-regulated and proteostasis-related transcription factors appeared to govern gene expression changes across cell types at some sites of gH2AX. Finally, we found that glia have a robust transcriptional response to glucocorticoids and some of these genes are sites of brain DSBs. In Chapter 3, we examined the relationship between brain DNA breaks and TOP2B function. We utilized a mouse forebrain excitatory neuron-specific functional knockout of Top2b. We found that neuronal loss of Top2b in the medial prefrontal cortex (mPFC) impairs both the expression of long genes and gene induction following exposure to a learning paradigm. Ultimately, loss of Top2b leads to abnormal learning and cognition.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biology

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2021

Author dc:creator

• Stott, Ryan(Ryan Timothy)

Advisor dc:contributor.advisor

• Li-Huei Tsai.

• Biology.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/130669

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/130669