Engineering of tools for De Novo Assembly of Human Cells

Organs for transplantation has continuously been in short supply and, given COVID-19's propensity to adversely impact solid organs, the shortage will likely become exacerbated. For decades, the field of tissue engineering has developed innovative methods to generate model tissues de novo. Top-down approaches, such as microfluidics and 3D bioprinting, provide spatial control by patterning cell types with high resolution, but face challenges in reproducing physiologically accurate cell types and interactions. Bottom-up methods, such as organoids, induce pluripotent cells to differentiate into aggregates that resemble their in vivo counterparts, yet the size and complexity of these structures are limited by nutrient diffusion and the morphology cannot be controlled. An ideal system would allow for high spatial control while retaining native cell-cell interactions formed through developmental progression.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Harvard University--MIT Division of Health Sciences and Technology

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Chao, Chung-Yun(Chung-Yun George)

Advisor dc:contributor.advisor

• George M. Church,.

• Harvard--MIT Program in Health Sciences and Technology.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/130205

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/130205