Hierarchical selective electrokinetic concentration : the universal next-generation biomolecule enrichment technique for molecular diagnostics

Rapid and reliable detection of ultralow-abundance nucleic acids and proteins in complex biological media may greatly advance clinical diagnostics and biotechnology development. Because of the slow mass transport and weak binding kinetics at ultralow concentration of target biomolecules, enrichment of target biomolecules plays an essential role in the detection of ultralow-abundance biomolecules. Currently, nucleic acid tests rely on enzymatic processes for target amplification (e.g. polymerase chain reaction), which have many inherent issues restricting their implementation in diagnostics. On the other hand, there exist no protein amplification techniques, greatly limiting the development of protein-based diagnosis.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Ouyang, Wei,Ph.D.Massachusetts Institute of Technology.

Advisor dc:contributor.advisor

• Jongyoon Han.

• Electrical Engineering and Computer Science.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/128323

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/128323