Generating single-domain antibodies against fibronectin splice variants

Here, I describe the process of generating single-domain antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of tumor samples, and antibody libraries were generated. Using these libraries to pan against, I selected for VHH which bind to EIIIA and EIIIB. Since these splice variants are upregulated in tumor angiogenesis and are rarely seen elsewhere in adult tissues, antibodies targeting EIIIA or EIIIB may be of use for imaging tumors and metastases.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biology.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2017

Author dc:creator

• Peterson, Alexis W

Advisor dc:contributor.advisor

• Richard Hynes.

• Biology.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/108893

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/108893