Core-based hyperbranched polyethyleneimine coating in capillary electrophoresis

In capillary electrophoresis (CE), chemical and dynamic modification of a fused silica surface can be used to control the electroosmotic flow (EOF), to minimize the interaction of analytes with the capillary surface and to modify the selectivity. , core-based hyperbranched polyethyleneimine (CHPEI) polymer utilized as either a static or dynamic coating in this study. A CHPEI-coated capillary was simply constructed in a rinsing fashion or by adding a small amount of CHPEI in a running buffer. In CHPEI-coated capillaries, several parameters were studied as follows: (1) EOF as a function of buffer pH; (2) effect of coating media (NaCl solution) concentration; (3) effect of buffer concentration; and (4) stability and reproducibility of the coating. Since CHPEI25-coated capillary provides greater EOF stability, CHPEI25 was chosen for further studies. The performance of CHPEI25 dynamic-, static-, and hybrid-coated capillaries was investigated in the separations of phenols, basic amino acids, B vitamins, aniline and its derivatives. The separation performance of CHPEI25 dynamic-coated capillaries were also compared to that of commercial eCAPTM capillary from Beckman Coulter. It is evident that CHPEI25 dynamic-coated capillary provides significantly improved peak resolution under identical separation conditions compared to that in eCAPTM.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Chemistry (MU)

Grantor dc:publisher

University of Missouri--Columbia

Year dc:date.issued

2009

Author dc:creator

• Boonyakong, Cheerapa, 1972-

Advisor dc:contributor.advisor

• Tucker, Sheryl A. (Sheryl Ann)

Statement dc:rights

• OpenAccess.

Language dc:language.iso

eng, English

DOI

https://doi.org/10.32469/10355/6145

OAI identifier oai:identifier

oai:mospace.umsystem.edu:10355/6145