{"id":{"repo_id":"missouri","oai_identifier":"oai:mospace.umsystem.edu:10355/6145"},"canonical_url":"https://search.dev.ndltd.org/etd/missouri/oai:mospace.umsystem.edu:10355/6145","repository":{"repo_id":"missouri","name":"University of Missouri","base_url":"https://mospace.umsystem.edu/oai/request"},"display":{"title":"Core-based hyperbranched polyethyleneimine coating in capillary electrophoresis","abstract":"In capillary electrophoresis (CE), chemical and dynamic modification of a fused silica surface can be used to control the electroosmotic flow (EOF), to minimize the interaction of analytes with the capillary surface and to modify the selectivity. , core-based hyperbranched polyethyleneimine (CHPEI) polymer utilized as either a static or dynamic coating in this study. A CHPEI-coated capillary was simply constructed in a rinsing fashion or by adding a small amount of CHPEI in a running buffer. In CHPEI-coated capillaries, several parameters were studied as follows: (1) EOF as a function of buffer pH; (2) effect of coating media (NaCl solution) concentration; (3) effect of buffer concentration; and (4) stability and reproducibility of the coating. Since CHPEI25-coated capillary provides greater EOF stability, CHPEI25 was chosen for further studies. The performance of CHPEI25 dynamic-, static-, and hybrid-coated capillaries was investigated in the separations of phenols, basic amino acids, B vitamins, aniline and its derivatives. The separation performance of CHPEI25 dynamic-coated capillaries were also compared to that of commercial eCAPTM capillary from Beckman Coulter. It is evident that CHPEI25 dynamic-coated capillary provides significantly improved peak resolution under identical separation conditions compared to that in eCAPTM.","abstract_html":"In capillary electrophoresis (CE), chemical and dynamic modification of a fused silica surface can be used to control the electroosmotic flow (EOF), to minimize the interaction of analytes with the capillary surface and to modify the selectivity. , core-based hyperbranched polyethyleneimine (CHPEI) polymer utilized as either a static or dynamic coating in this study. A CHPEI-coated capillary was simply constructed in a rinsing fashion or by adding a small amount of CHPEI in a running buffer. In CHPEI-coated capillaries, several parameters were studied as follows: (1) EOF as a function of buffer pH; (2) effect of coating media (NaCl solution) concentration; (3) effect of buffer concentration; and (4) stability and reproducibility of the coating. Since CHPEI25-coated capillary provides greater EOF stability, CHPEI25 was chosen for further studies. The performance of CHPEI25 dynamic-, static-, and hybrid-coated capillaries was investigated in the separations of phenols, basic amino acids, B vitamins, aniline and its derivatives. The separation performance of CHPEI25 dynamic-coated capillaries were also compared to that of commercial eCAPTM capillary from Beckman Coulter. It is evident that CHPEI25 dynamic-coated capillary provides significantly improved peak resolution under identical separation conditions compared to that in eCAPTM.","abstract_has_math":false,"creators":["Boonyakong, Cheerapa, 1972-"],"institution":"University of Missouri--Columbia","degree_name":"Ph. D.","degree_level":"Doctoral","degree_discipline":"Chemistry (MU)","degree_department":null,"school":null,"contributors":[],"advisors":["Tucker, Sheryl A. 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The performance of CHPEI25 dynamic-, static-, and hybrid-coated capillaries was investigated in the separations of phenols, basic amino acids, B vitamins, aniline and its derivatives. The separation performance of CHPEI25 dynamic-coated capillaries were also compared to that of commercial eCAPTM capillary from Beckman Coulter. It is evident that CHPEI25 dynamic-coated capillary provides significantly improved peak resolution under identical separation conditions compared to that in eCAPTM."]},{"key":"dc:title","label":"Title","values":["Core-based hyperbranched polyethyleneimine coating in capillary electrophoresis"]}]}],"canonical_facts":{"dc:contributor.advisor":["Tucker, Sheryl A. 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A CHPEI-coated capillary was simply constructed in a rinsing fashion or by adding a small amount of CHPEI in a running buffer. In CHPEI-coated capillaries, several parameters were studied as follows: (1) EOF as a function of buffer pH; (2) effect of coating media (NaCl solution) concentration; (3) effect of buffer concentration; and (4) stability and reproducibility of the coating. Since CHPEI25-coated capillary provides greater EOF stability, CHPEI25 was chosen for further studies. The performance of CHPEI25 dynamic-, static-, and hybrid-coated capillaries was investigated in the separations of phenols, basic amino acids, B vitamins, aniline and its derivatives. The separation performance of CHPEI25 dynamic-coated capillaries were also compared to that of commercial eCAPTM capillary from Beckman Coulter. 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