The developmental liver transcriptome of Rattus norvegicus

Our research is involved in the transcriptional dynamics of perinatal liver development. The transcription factors and their downstream effects have been well studied in regards to early liver development, yet perinatal liver development is much less well understood. In an attempt to investigate this area of research, we developed a time course experiment including both pre- and postnatal liver samples whereby RNA sequencing was performed on each of the biological stages. We investigated such areas as gene expression, isoform expression, transcriptional regulation, and post-transcriptional modifications of transcribed molecules. Furthermore, we used a bioinformatics approach to improve upon the reference annotation for known genes. Our research gives insight to the biological phenomenon of late gestational and post parturition liver development. Additionally, we provided annotation for over 1000 regions in the rat reference genome. Further work will include an attempt to investigate novel transcript and gene discovery, as well as an attempt to characterize the extent of RNA editing in perinatal liver development.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Animal sciences (MU)

Grantor dc:publisher

University of Missouri--Columbia

Year dc:date.issued

2012

Author dc:creator

• Chapple, Richard Harrison

Advisors dc:contributor.advisor

• Taylor, J. (Jerry)
• Schnabel, Robert

• liver development
• transcription factor
• gene expression
• reference genome

Statement dc:rights

• OpenAccess.

Language dc:language.iso

eng, English

Handle dc:identifier.uri

http://hdl.handle.net/10355/15244

OAI identifier oai:identifier

oai:mospace.umsystem.edu:10355/15244