In vitro and in vivo approaches to tumor targeting by phage display

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Approximately 30% of breast cancers are linked with the over-expression of ErbB2. Because of its critical role in signal transduction, the progression of breast cancer and its localization on the surface of several types of cancer cells, ErbB2 is a commonly used as a target for the search for protein and peptide ligands that may be useful as therapeutic and diagnostic agents for treating and imaging cancer. In the work described here, I used several commonly used in vitro, ex vivo, and in vivo phage display techniques and binding assays in an attempt to discover such peptides. This work lead to the discovery a phage mutant which interferes with the usage of the phage display libraries of the type used during this research. In addition, I also report the results of a quantitative assessment of a new enzymatically releasable phage construct.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Biological sciences (MU)

Grantor dc:publisher

University of Missouri--Columbia

Year dc:date.issued

2010

Author dc:creator

• Thomas, William Donald, 1974-

Advisor dc:contributor.advisor

• Smith, George P., 1941-

Statement dc:rights

• Access to files is limited to the campuses of the University of Missouri with SSO login.

Language dc:language.iso

eng, English

DOI

https://doi.org/10.32469/10355/10348

OAI identifier oai:identifier

oai:mospace.umsystem.edu:10355/10348